| pubmed-article:17103370 | pubmed:abstractText | The enhancement of Spodoptera litura (F.) nucleopolyhedrovirus (SlNPV) activity using the chitin synthesis inhibitor chlorfluazuron was investigated. When tested against fifth-instar S. litura larvae, chlorfluazuron produced synergistic effects at doses of 0.05 and 0.025 microg per insect, and additive effects at doses of 0.1 and 0.2 microg. Furthermore, the time required for SlNPV to kill larvae was significantly reduced by chlorfluazuron at all doses tested. The activity and killing speed of Autographa californica (Spey) nucleopolyhedrovirus (AcMNPV) against third-instar Spodoptera exigua (Hübner) larvae were similarly improved by chlorfluazuron at a dose of 0.05 microg per larva. Furthermore, the growth of S. exigua was significantly retarded by chlorfluazuron. Environmental scanning electron microscopy (ESEM) showed that the peritrophic matrices (PMs) of S. litura exposed to chlorfluazuron alone, or the combination treatment, were markedly disrupted. Obvious ruptures on the outer surfaces of the PM were observed, which potentially facilitated the passage of virions through the matrix. | lld:pubmed |
