| pubmed-article:17087820 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17087820 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:17087820 | lifeskim:mentions | umls-concept:C0019707 | lld:lifeskim |
| pubmed-article:17087820 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:17087820 | lifeskim:mentions | umls-concept:C0443288 | lld:lifeskim |
| pubmed-article:17087820 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:17087820 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:17087820 | lifeskim:mentions | umls-concept:C1311670 | lld:lifeskim |
| pubmed-article:17087820 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:17087820 | pubmed:dateCreated | 2006-11-13 | lld:pubmed |
| pubmed-article:17087820 | pubmed:abstractText | In order to characterize the antiviral activity of human TRIM5alpha in more detail human derived indicator cell lines over expressing wild type human TRIM5alpha were generated and challenged with HIV-1 and HIV-2 viruses pseudotyped with HIV envelope proteins in comparison to VSV-G pseudotyped particles. HIV envelope protein pseudotyped particles (HIV-1[NL4.3], HIV-1[BaL]) showed a similar restriction to infection (12 fold inhibition) compared to VSV-G pseudotyped viruses after challenging TZM-huTRIM5alpha cells. For HIV-2 a stronger restriction to infection was observed when the homologous envelope protein Env42S was pseudotyped onto these particles compared to VSV-G pseudotyped HIV-2 particles (8.6 fold inhibition versus 3.4 fold inhibition). It has been shown that HIV-2 is restricted by the restriction factor Lv2, acting on capsid like TRIM5alpha. A mutation of amino acid 73 (I73V) of HIV-2 capsid renders this virus Lv2-insensitive. Lv2-insensitive VSV-G pseudotyped HIV-2/I73V particles showed a similar restriction to infection as did HIV-2[VSV-G] particles (4 fold inhibition). HIV-2 envelope protein (Env42S)-pseudotyped HIV-2/I73V particles revealed a 9.3 fold increase in infection in TZM cells but remained restricted in TZM-huTRIM5alpha cells (80.6 fold inhibition) clearly indicating that at least two restriction factors, TRIM5alpha and Lv2, act on incoming HIV-2 particles. Further challenge experiments using primary isolates from different HIV-1 subtypes and from HIV-1 group O showed that wild type human TRIM5alpha restricted infection independent of coreceptor use of the infecting particle but to variable degrees (between 1.2 and 19.6 fold restriction). | lld:pubmed |
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| pubmed-article:17087820 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:17087820 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:17087820 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17087820 | pubmed:issn | 1742-4690 | lld:pubmed |
| pubmed-article:17087820 | pubmed:author | pubmed-author:DittmarMatthi... | lld:pubmed |
| pubmed-article:17087820 | pubmed:author | pubmed-author:KaumannsPatri... | lld:pubmed |
| pubmed-article:17087820 | pubmed:author | pubmed-author:HagmannIsabel... | lld:pubmed |
| pubmed-article:17087820 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:17087820 | pubmed:volume | 3 | lld:pubmed |
| pubmed-article:17087820 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17087820 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17087820 | pubmed:pagination | 79 | lld:pubmed |
| pubmed-article:17087820 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:17087820 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:17087820 | pubmed:articleTitle | Human TRIM5alpha mediated restriction of different HIV-1 subtypes and Lv2 sensitive and insensitive HIV-2 variants. | lld:pubmed |
| pubmed-article:17087820 | pubmed:affiliation | Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany. patrick.kaumanns@med.uni-heidelberg.de | lld:pubmed |
| pubmed-article:17087820 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17087820 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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