pubmed-article:1706566 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0031809 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0037657 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0074825 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0202202 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0037473 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0242210 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0023688 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0439810 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C1704241 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C1550605 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0456205 | lld:lifeskim |
pubmed-article:1706566 | lifeskim:mentions | umls-concept:C0443131 | lld:lifeskim |
pubmed-article:1706566 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:1706566 | pubmed:dateCreated | 1991-4-25 | lld:pubmed |
pubmed-article:1706566 | pubmed:abstractText | The possibility that sodium dodecyl sulfate (SDS)-stable complexes of insulin-like growth factor I (IGF-I) and its binding proteins (IGF-BP) exist in rat serum has been examined by using SDS-polyacrylamide gel electrophoresis (PAGE) followed by both [125I]IGF-I ligand blotting and immunoblotting with antisera directed against either IGF-BP3 or IGF-I. While ligand blotting of rat serum only revealed free IGF-BP subunits (Mr approximately 50, 35, and 30 kDa), immunoblotting with either the IGF-BP3 antiserum or IGF-I antiserum revealed major immunoreactive bands with higher molecular weights (greater than 110, approximately 100, and approximately 84 kDa). The IGF-BP3 antiserum also stained the 50-kDa form of the serum IGF-BP. Specifically stained protein bands were identified by comparison with control immunoblots incubated with normal rabbit serum. Treating the serum with 0.1 N HCl prior to electrophoresis reduced the amount of high molecular weight IGF-BP3 immunoreactive species, with a concomitant increase in the amount of the 50-kDa form. A similar result was obtained if the samples were boiled prior to electrophoresis. These data indicate that not all IGF-BP/IGF complexes may dissociate under normal SDS-PAGE conditions. Therefore, data obtained by using ligand blotting alone may underestimate the amount of total IGF-BP present, especially if the mixture being analyzed also contains large amounts of IGF. | lld:pubmed |
pubmed-article:1706566 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:language | eng | lld:pubmed |
pubmed-article:1706566 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:1706566 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1706566 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1706566 | pubmed:month | Nov | lld:pubmed |
pubmed-article:1706566 | pubmed:issn | 0003-2697 | lld:pubmed |
pubmed-article:1706566 | pubmed:author | pubmed-author:LindKK | lld:pubmed |
pubmed-article:1706566 | pubmed:author | pubmed-author:NakataniAA | lld:pubmed |
pubmed-article:1706566 | pubmed:author | pubmed-author:ShimonakaMM | lld:pubmed |
pubmed-article:1706566 | pubmed:author | pubmed-author:BicsakT ATA | lld:pubmed |
pubmed-article:1706566 | pubmed:author | pubmed-author:MalkowskiMM | lld:pubmed |
pubmed-article:1706566 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1706566 | pubmed:day | 15 | lld:pubmed |
pubmed-article:1706566 | pubmed:volume | 191 | lld:pubmed |
pubmed-article:1706566 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1706566 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1706566 | pubmed:pagination | 75-9 | lld:pubmed |
pubmed-article:1706566 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:meshHeading | pubmed-meshheading:1706566-... | lld:pubmed |
pubmed-article:1706566 | pubmed:year | 1990 | lld:pubmed |
pubmed-article:1706566 | pubmed:articleTitle | Insulin-like growth factor binding protein measurement: sodium dodecyl sulfate-stable complexes with insulin-like growth factor in serum prevent accurate assessment of total binding protein content by ligand blotting. | lld:pubmed |
pubmed-article:1706566 | pubmed:affiliation | Whittier Institute for Diabetes and Endocrinology, Department of Molecular Endocrinology, La Jolla, California 92037. | lld:pubmed |
pubmed-article:1706566 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1706566 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |