| pubmed-article:17055527 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17055527 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:17055527 | lifeskim:mentions | umls-concept:C1719039 | lld:lifeskim |
| pubmed-article:17055527 | lifeskim:mentions | umls-concept:C0449445 | lld:lifeskim |
| pubmed-article:17055527 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:17055527 | lifeskim:mentions | umls-concept:C2003942 | lld:lifeskim |
| pubmed-article:17055527 | lifeskim:mentions | umls-concept:C2352041 | lld:lifeskim |
| pubmed-article:17055527 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:17055527 | pubmed:dateCreated | 2006-12-1 | lld:pubmed |
| pubmed-article:17055527 | pubmed:abstractText | We present a real-time PCR approach for the identification and subtyping of HLA-DR4 alleles. The technique, which uses sequence-specific primers and probes in conjunction with real-time PCR for the detection and differentiation of target alleles, is rapid, involves minimal hands-on time, and is inexpensive compared to existing methods. Further, there is no post-PCR handling, so the risk of contamination is avoided. We have validated the assay using 44 blinded and 56 unblinded samples, which were identified with 100% accuracy, sensitivity, and specificity. We demonstrate the applicability of this assay as an alternative approach to traditional HLA typing methods. | lld:pubmed |
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| pubmed-article:17055527 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17055527 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17055527 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:17055527 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17055527 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:17055527 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:17055527 | pubmed:author | pubmed-author:NepomGerald... | lld:pubmed |
| pubmed-article:17055527 | pubmed:author | pubmed-author:GersukVivian... | lld:pubmed |
| pubmed-article:17055527 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17055527 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:17055527 | pubmed:volume | 317 | lld:pubmed |
| pubmed-article:17055527 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17055527 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17055527 | pubmed:pagination | 64-70 | lld:pubmed |
| pubmed-article:17055527 | pubmed:dateRevised | 2010-12-3 | lld:pubmed |
| pubmed-article:17055527 | pubmed:meshHeading | pubmed-meshheading:17055527... | lld:pubmed |
| pubmed-article:17055527 | pubmed:meshHeading | pubmed-meshheading:17055527... | lld:pubmed |
| pubmed-article:17055527 | pubmed:meshHeading | pubmed-meshheading:17055527... | lld:pubmed |
| pubmed-article:17055527 | pubmed:meshHeading | pubmed-meshheading:17055527... | lld:pubmed |
| pubmed-article:17055527 | pubmed:meshHeading | pubmed-meshheading:17055527... | lld:pubmed |
| pubmed-article:17055527 | pubmed:meshHeading | pubmed-meshheading:17055527... | lld:pubmed |
| pubmed-article:17055527 | pubmed:meshHeading | pubmed-meshheading:17055527... | lld:pubmed |
| pubmed-article:17055527 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:17055527 | pubmed:articleTitle | A real-time PCR approach for rapid high resolution subtyping of HLA-DRB1*04. | lld:pubmed |
| pubmed-article:17055527 | pubmed:affiliation | Benaroya Research Institute at Virginia Mason, 1201 9th Ave., Seattle, WA 98101, USA. vgersuk@benaroyaresearch.org | lld:pubmed |
| pubmed-article:17055527 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17055527 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17055527 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| pubmed-article:17055527 | pubmed:publicationType | Validation Studies | lld:pubmed |
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