| pubmed-article:17049932 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17049932 | lifeskim:mentions | umls-concept:C1123023 | lld:lifeskim |
| pubmed-article:17049932 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:17049932 | lifeskim:mentions | umls-concept:C0014520 | lld:lifeskim |
| pubmed-article:17049932 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:17049932 | lifeskim:mentions | umls-concept:C2004454 | lld:lifeskim |
| pubmed-article:17049932 | lifeskim:mentions | umls-concept:C0205174 | lld:lifeskim |
| pubmed-article:17049932 | lifeskim:mentions | umls-concept:C0332183 | lld:lifeskim |
| pubmed-article:17049932 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:17049932 | pubmed:dateCreated | 2007-1-2 | lld:pubmed |
| pubmed-article:17049932 | pubmed:abstractText | Mutations of the Xpc gene cause a deficiency in global genome repair, a subpathway of nucleotide excision repair (NER), in mammalian cells. We used transgenic mice harboring the lambda-phage-based lacZ mutational reporter gene to study the effect of an Xpc null mutation (Xpc-/-) on damage induction, repair and mutagenesis in mouse skin epidermis after UVB irradiation. UVB induced equal amounts of cyclobutane pyrimidine dimers (CPDs) and pyrimidine(6-4)pyrimidone photoproducts (64PPs) in mouse skin epidermis of Xpc-/- and wild-type mice. CPDs were not significantly removed in either of the mouse genotypes by 12h after irradiation, whereas removal of 64PPs was observed in the wild-type. Irradiation with 300 and 400J/m2 UVB increased the lacZ mutant frequency in the Xpc-/- epidermis to at least twice as high as in the wild-type. Ninety-nine lacZ mutants isolated from the UVB-exposed epidermis of Xpc(-/-)mice were analyzed and compared with mutant sequences from irradiated wild-type mice. The spectra of the mutations in the two genotypes were both highly UV-specific and similar in the dominance of C-->T transitions at dipyrimidine sites; however, Xpc-/- mice had a higher frequency of two-base tandem substitutions, including CC-->TT mutations, three-base tandem substitutions and double base substitutions that were separated by one unchanged base in a three-base sequence (alternating mutations). These tandem/alternating mutations included a remarkably large number of triplet mutations, a recently reported, novel type of UV-specific mutation, characterized by multiple base substitutions or frameshifts within a three-nucleotide sequence containing a dipyrimidine. We concluded that the triplet mutation is a UV-specific mutation that preferably occurs in NER deficient genetic backgrounds. | lld:pubmed |
| pubmed-article:17049932 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17049932 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17049932 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17049932 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17049932 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17049932 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17049932 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:17049932 | pubmed:issn | 1568-7864 | lld:pubmed |
| pubmed-article:17049932 | pubmed:author | pubmed-author:MoriToshioT | lld:pubmed |
| pubmed-article:17049932 | pubmed:author | pubmed-author:NikaidoOsamuO | lld:pubmed |
| pubmed-article:17049932 | pubmed:author | pubmed-author:OnoTetsuyaT | lld:pubmed |
| pubmed-article:17049932 | pubmed:author | pubmed-author:IkehataHirono... | lld:pubmed |
| pubmed-article:17049932 | pubmed:author | pubmed-author:SaitoYusukeY | lld:pubmed |
| pubmed-article:17049932 | pubmed:author | pubmed-author:YanaseFumitak... | lld:pubmed |
| pubmed-article:17049932 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17049932 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:17049932 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:17049932 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17049932 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17049932 | pubmed:pagination | 82-93 | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:meshHeading | pubmed-meshheading:17049932... | lld:pubmed |
| pubmed-article:17049932 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17049932 | pubmed:articleTitle | Frequent recovery of triplet mutations in UVB-exposed skin epidermis of Xpc-knockout mice. | lld:pubmed |
| pubmed-article:17049932 | pubmed:affiliation | Department of Cell Biology, Graduate School of Medicine, Tohoku University, Sendai 980-8575, Japan. ikehata@mail.tains.tohoku.ac.jp | lld:pubmed |
| pubmed-article:17049932 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17049932 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:22591 | entrezgene:pubmed | pubmed-article:17049932 | lld:entrezgene |
