| pubmed-article:1703445 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C1552132 | lld:lifeskim |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C0004793 | lld:lifeskim |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C1419397 | lld:lifeskim |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:1703445 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:1703445 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1703445 | pubmed:dateCreated | 1991-3-6 | lld:pubmed |
| pubmed-article:1703445 | pubmed:abstractText | Eukaryotic 5S rRNA hybridizes specifically with 18S rRNA in vitro to form a stable intermolecular RNA:RNA hybrid. We have used 5S rRNA/18S rRNA fragment hybridization studies coupled with ribonuclease digestion and primer extension/chain termination analysis of 5S rRNA:18S rRNA hybrids to more completely map those mouse 5S rRNA and 18S rRNA sequences responsible for duplex formation. Fragment hybridization analysis has defined a 5'-terminal region of 5S rRNA (nucleotides 6-27) which base-pairs with two independent sequences in 18S rRNA designated Regions 1 (nucleotides 1157-1180) and 2 (nucleotides 1324-1339). Ribonuclease digestion of isolated 5S rRNA:18S rRNA hybrids with both single-strand- and double-strand-specific nucleases supports the involvement of this 5'-terminal 5S rRNA sequence in 18S rRNA hybridization. Primer extension/chain termination analysis of isolated 5S rRNA:18S rRNA hybrids confirms the base-pairing of 5S rRNA to the designated Regions 1 and 2 of 18S rRNA. Using these results, 5S rRNA:18S rRNA intermolecular hybrid structures are proposed. Comparative sequence analysis revealed the conservation of these hybrid structures in higher eukaryotes and the same but smaller core hybrid structures in lower eukaryotes and prokaryotes. This suggests that the 5S rRNA:16S/18S rRNA hybrids have been conserved in evolution for ribosome function. | lld:pubmed |
| pubmed-article:1703445 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703445 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703445 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1703445 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703445 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1703445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703445 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1703445 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:1703445 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:1703445 | pubmed:author | pubmed-author:MaxwellE SES | lld:pubmed |
| pubmed-article:1703445 | pubmed:author | pubmed-author:SargeK DKD | lld:pubmed |
| pubmed-article:1703445 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1703445 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:1703445 | pubmed:volume | 1088 | lld:pubmed |
| pubmed-article:1703445 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1703445 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1703445 | pubmed:pagination | 57-70 | lld:pubmed |
| pubmed-article:1703445 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:meshHeading | pubmed-meshheading:1703445-... | lld:pubmed |
| pubmed-article:1703445 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1703445 | pubmed:articleTitle | Intermolecular hybridization of 5S rRNA with 18S rRNA: identification of a 5'-terminally-located nucleotide sequence in mouse 5S rRNA which base-pairs with two specific complementary sequences in 18S rRNA. | lld:pubmed |
| pubmed-article:1703445 | pubmed:affiliation | Department of Biochemistry, North Carolina State University, Raleigh 27695-7622. | lld:pubmed |
| pubmed-article:1703445 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1703445 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1703445 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1703445 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1703445 | lld:pubmed |
