| pubmed-article:1703216 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C0032043 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C0041178 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C0001128 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C0021747 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C0205265 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:1703216 | lifeskim:mentions | umls-concept:C1555582 | lld:lifeskim |
| pubmed-article:1703216 | pubmed:dateCreated | 1991-2-27 | lld:pubmed |
| pubmed-article:1703216 | pubmed:abstractText | Human placental trophoblast interferon (tro-IFN), induced in trophoblast cultures by a superinduction procedure, was purified to a homogeneous product with retention of biological activity. The problems associated with isolation from serum-containing medium were overcome by a combination of Blue Sepharose affinity chromatography and reversed-phase HPLC (RP-HPLC) on Separon SGX C-18. This two-step purification procedure yielded tro-IFN with a specific activity of 3.4 x 10(7) international units/mg of protein. The overall recovery of interferon activity was 66.7%. The purified tro-IFN was shown to be a glycoprotein with an Mr of 24K on native and SDS-PAGE. Its antiviral activity was stable at pH 2.0 at 37 degrees C but was sensitive to heat at 56 degrees C for 1 h and was neutralized by antibodies to human IFN-beta. | lld:pubmed |
| pubmed-article:1703216 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1703216 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703216 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1703216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1703216 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1703216 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1703216 | pubmed:issn | 0022-1317 | lld:pubmed |
| pubmed-article:1703216 | pubmed:author | pubmed-author:TóthF DFD | lld:pubmed |
| pubmed-article:1703216 | pubmed:author | pubmed-author:EbbesenPP | lld:pubmed |
| pubmed-article:1703216 | pubmed:author | pubmed-author:Nørskov-Lauri... | lld:pubmed |
| pubmed-article:1703216 | pubmed:author | pubmed-author:PetersenP MPM | lld:pubmed |
| pubmed-article:1703216 | pubmed:author | pubmed-author:Aboagye-Mathi... | lld:pubmed |
| pubmed-article:1703216 | pubmed:author | pubmed-author:JuhlCC | lld:pubmed |
| pubmed-article:1703216 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1703216 | pubmed:volume | 71 ( Pt 12) | lld:pubmed |
| pubmed-article:1703216 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1703216 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1703216 | pubmed:pagination | 3061-6 | lld:pubmed |
| pubmed-article:1703216 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:meshHeading | pubmed-meshheading:1703216-... | lld:pubmed |
| pubmed-article:1703216 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1703216 | pubmed:articleTitle | Purification and initial characterization of human placental trophoblast interferon induced by polyriboinosinic.polyribocytidylic acid. | lld:pubmed |
| pubmed-article:1703216 | pubmed:affiliation | Danish Cancer Society, Department of Virus and Cancer, Aarhus, Denmark. | lld:pubmed |
| pubmed-article:1703216 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1703216 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1703216 | lld:pubmed |
