| pubmed-article:16997219 | pubmed:abstractText | When guanine and uracil form hydrogen bonds in the pairing scheme first proposed by Crick one would expect that poly(A,G) will form an unperturbed double helix with poly U at room temperature in a dilute electrolyte solution (0.1 M NaCl). We have demonstrated by Raman- and IR-spectroscopy that the secondary structure of poly(A.G) . poly U is very similar to the structure of poly A . poly U; only the thermal stability of the double helix seems slightly lower than the stability of poly A . poly U, whereas the average helix length is unaffected by the dispersed G . U base pairs. From our input ratio of guanine and adenine we estimate that about every fourth base pair is a wobble pair. | lld:pubmed |
