| pubmed-article:16978869 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C0007765 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C0596402 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C0017822 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C1515844 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C0244104 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:16978869 | lifeskim:mentions | umls-concept:C0205248 | lld:lifeskim |
| pubmed-article:16978869 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16978869 | pubmed:dateCreated | 2006-10-23 | lld:pubmed |
| pubmed-article:16978869 | pubmed:abstractText | Parkinson disease (PD) is the second-most common age-related neurodegenerative disease and is characterized by the selective destruction of dopaminergic neurons. Increasing evidence indicates that oxidative stress plays a crucial role in the pathogenesis of idiopathic PD. Anti-oxidant agents including catalase, manganese porphyrin and pyruvate confer cytoprotection to different cell cultures when challenged with 6-hydroxydopamine (6-OHDA). Herein we used rat cerebellar granular cell cultures to ascertain the plausible cellular pathways involved in pyruvate-induced cytoprotection against 0.1 mM 6-OHDA. Pyruvate provided cytoprotection in a concentration-dependent manner (2-10 mM). Consistent with its well-established anti-oxidant capacity, pyruvate (10 mM) prevented 6-OHDA-induced lipid peroxidation by blocking the rise in intracellular peroxides and maintaining the intracellular reduced glutathione (GSH) levels. Further experiments revealed that pyruvate increased Akt, but not extracellular signal-regulated kinase phosphorylation. Moreover, phosphatidylinositol 3-kinase (PI3K) inhibitors attenuated pyruvate-induced cytoprotection indicating that PI3K-mediated Akt activation is necessary for pyruvate to induce cytoprotection. On the other hand, pyruvate also up-regulated glutathione peroxidase mRNA levels, but not those of the anti-oxidant enzymes superoxide dismutase-1 and -2, catalase or the anti-apoptotic oncogenes Bcl-2 or Bcl-xL. In summary, our results strongly suggest that pyruvate, besides the anti-oxidant properties related to its structure, exerts cytoprotective actions by activating different anti-apoptotic routes that include gene regulation and Akt pathway activation. | lld:pubmed |
| pubmed-article:16978869 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16978869 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16978869 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16978869 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:16978869 | pubmed:issn | 0969-9961 | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:CalvoSS | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:JordánJJ | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:GalindoM FMF | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:PastorM DMD | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:SolerR MRM | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:Gomez-LazaroM... | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:Fernandez-Gom... | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:Garcia-Martin... | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:Melero-Fernan... | lld:pubmed |
| pubmed-article:16978869 | pubmed:author | pubmed-author:Gou-FabregasM... | lld:pubmed |
| pubmed-article:16978869 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16978869 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:16978869 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16978869 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16978869 | pubmed:pagination | 296-307 | lld:pubmed |
| pubmed-article:16978869 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:16978869 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16978869 | pubmed:articleTitle | Pyruvate protects cerebellar granular cells from 6-hydroxydopamine-induced cytotoxicity by activating the Akt signaling pathway and increasing glutathione peroxidase expression. | lld:pubmed |
| pubmed-article:16978869 | pubmed:affiliation | Departamento de Ciencias Médicas, Facultad de Medicina, Universidad Castilla-La Mancha, and Servicio de Farmacia, Complejo Hospitalario Universitario de Albacete, Spain. | lld:pubmed |
| pubmed-article:16978869 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16978869 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:24185 | entrezgene:pubmed | pubmed-article:16978869 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:16978869 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16978869 | lld:pubmed |
