| pubmed-article:16970347 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C0025047 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C2756969 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C0302908 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C0185115 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C0205554 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C0870883 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C0037813 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:16970347 | lifeskim:mentions | umls-concept:C0220894 | lld:lifeskim |
| pubmed-article:16970347 | pubmed:issue | 18 | lld:pubmed |
| pubmed-article:16970347 | pubmed:dateCreated | 2006-9-14 | lld:pubmed |
| pubmed-article:16970347 | pubmed:abstractText | We developed an analytical method using off-line solid-phase extraction (SPE) coupled with on-line SPE and isotope-dilution high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to determine the concentrations of phthalate metabolites in human meconium and in semen. First, we used off-line SPE to remove interfering proteins and other biomolecules from the samples. Then, we preconcentrated the phthalate metabolites in the extract using on-line SPE before measuring them by HPLC-MS/MS. For most of the analytes, the limits of detection ranged between 0.2 and 0.7 ng/g for meconium and between 0.3 and 0.7 ng/mL for semen. The recovery after off-line SPE varied for most analytes between 65 and 99% at concentrations ranging from 3.0 to 30.0 ng/mL in semen and between 67 and 103% at concentrations ranging from 2.0 to 10.0 ng/mL in meconium. Precision measured by the relative standard deviation ranged from 3.2 to 19.1% for intraday and from 3.9 to 18.6% for interday. We validated this novel approach--which is applicable to other biological matrixes, including serum and breast milk--on spiked samples and on five meconium samples and one pooled semen sample from people with no known occupational exposure to phthalates. | lld:pubmed |
| pubmed-article:16970347 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16970347 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16970347 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16970347 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16970347 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16970347 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16970347 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16970347 | pubmed:issn | 0003-2700 | lld:pubmed |
| pubmed-article:16970347 | pubmed:author | pubmed-author:NeedhamLarry... | lld:pubmed |
| pubmed-article:16970347 | pubmed:author | pubmed-author:KatoKayokoK | lld:pubmed |
| pubmed-article:16970347 | pubmed:author | pubmed-author:CalafatAntoni... | lld:pubmed |
| pubmed-article:16970347 | pubmed:author | pubmed-author:SilvaManori... | lld:pubmed |
| pubmed-article:16970347 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16970347 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:16970347 | pubmed:volume | 78 | lld:pubmed |
| pubmed-article:16970347 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16970347 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16970347 | pubmed:pagination | 6651-5 | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:meshHeading | pubmed-meshheading:16970347... | lld:pubmed |
| pubmed-article:16970347 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16970347 | pubmed:articleTitle | Quantifying phthalate metabolites in human meconium and semen using automated off-line solid-phase extraction coupled with on-line SPE and isotope-dilution high-performance liquid chromatography--tandem mass spectrometry. | lld:pubmed |
| pubmed-article:16970347 | pubmed:affiliation | Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA. | lld:pubmed |
| pubmed-article:16970347 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16970347 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16970347 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16970347 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16970347 | lld:pubmed |
