| pubmed-article:16941179 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C0238703 | lld:lifeskim |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C0012860 | lld:lifeskim |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C0015259 | lld:lifeskim |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C0518031 | lld:lifeskim |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C0205217 | lld:lifeskim |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C0439590 | lld:lifeskim |
| pubmed-article:16941179 | lifeskim:mentions | umls-concept:C1524003 | lld:lifeskim |
| pubmed-article:16941179 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16941179 | pubmed:dateCreated | 2006-9-7 | lld:pubmed |
| pubmed-article:16941179 | pubmed:abstractText | Recent research has demonstrated that lymphocyte apoptosis sensitivity appears to be related to training status and exercise intensity. This work investigated the effect of prolonged, submaximal treadmill running on percentage (%) apoptosis, % necrosis and DNA strand breaks in lymphocytes and related these to changes in total lymphocyte and blood cortisol concentrations in well-trained runners. Venous blood samples (n = 14) were taken immediately before (PRE), immediately after (IPE) and 3 h after (3PE) 2.5 h of treadmill running at 75% of VO2 max from eight well-trained male endurance athletes (age 34.2 +/- 2.44 years) and analysed for cellular content and serum cortisol concentrations. Lymphocytes were isolated from whole blood and % apoptotic and necrotic cell were detected by flow cytometry using Annexin V-FITC and propidium iodide uptake. DNA strand breaks were measured by single-cell gel electrophoresis. Despite a significant (P < 0.001) exercise-induced increase in mean serum cortisol concentrations and reduction in lymphocyte counts, the mean % Annexin-V positive cells (13.3 +/- 6.78 in PRE, 11.3 +/- 5.51 in IPE and 12.8 +/- 6.75 in 3PE samples) were not significantly different at the three time-points (P > 0.05). Mean DNA strand breaks in the lymphocytes also did not change significantly (P > 0.05) rising from 25.7 +/- 2.16 to 26.9 +/- 1.89 and 27.1 +/- 1.38 microm in IPE and 3PE samples, respectively. The exercise-induced changes in total blood lymphocyte counts and cortisol concentrations did not result in a significant change in % apoptotic lymphocytes or DNA strand breaks in the endurance-trained athletes during this prolonged, submaximal exercise. | lld:pubmed |
| pubmed-article:16941179 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16941179 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16941179 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16941179 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16941179 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16941179 | pubmed:issn | 1439-6319 | lld:pubmed |
| pubmed-article:16941179 | pubmed:author | pubmed-author:TylerNN | lld:pubmed |
| pubmed-article:16941179 | pubmed:author | pubmed-author:Van EdenMM | lld:pubmed |
| pubmed-article:16941179 | pubmed:author | pubmed-author:PetersE MEM | lld:pubmed |
| pubmed-article:16941179 | pubmed:author | pubmed-author:ChuturgoonA... | lld:pubmed |
| pubmed-article:16941179 | pubmed:author | pubmed-author:RamautarAA | lld:pubmed |
| pubmed-article:16941179 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16941179 | pubmed:volume | 98 | lld:pubmed |
| pubmed-article:16941179 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16941179 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16941179 | pubmed:pagination | 124-31 | lld:pubmed |
| pubmed-article:16941179 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:meshHeading | pubmed-meshheading:16941179... | lld:pubmed |
| pubmed-article:16941179 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16941179 | pubmed:articleTitle | Prolonged exercise does not cause lymphocyte DNA damage or increased apoptosis in well-trained endurance athletes. | lld:pubmed |
| pubmed-article:16941179 | pubmed:affiliation | School of Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, 519, Umbilo Road, Congella 4013, Private Bag 7, Durban, South Africa. futree@ukzn.ac.za | lld:pubmed |
| pubmed-article:16941179 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16941179 | pubmed:publicationType | Clinical Trial | lld:pubmed |
