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pubmed-article:16936283pubmed:abstractTextBased on data developed with the use of isolated lipid droplets from neonatal rat lung lipofibroblasts, we speculated previously that the droplet coat protein, adipose differentiation-related protein (ADFP), mediated the transfer of lipids into type 2 lung epithelial cells for the production of surfactant phospholipids. The present studies were designed to test the role of ADFP in this transfer with the use of ADFP-coated lipid droplets from CHO fibroblast cells and a cultured human lung epithelial cell line. We found no role for ADFP in the lipid transfer and conclude that a lipase associated with the lipid droplets hydrolyzes their core triacylglycerols, releasing fatty acids that are taken up by the epithelial cells.lld:pubmed
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pubmed-article:16936283pubmed:dateRevised2011-10-18lld:pubmed
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pubmed-article:16936283pubmed:articleTitleRole of adipose differentiation-related protein in lung surfactant production: a reassessment.lld:pubmed
pubmed-article:16936283pubmed:affiliationMembrane Regulation Section, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-8028, USA.lld:pubmed
pubmed-article:16936283pubmed:publicationTypeJournal Articlelld:pubmed
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