Linked Life Data

16927432

Source:http://linkedlifedata.com/resource/pubmed/id/16927432

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pubmed-article:16927432pubmed:abstractTextThe newly available techniques for sensitive proteome analysis and the resulting amount of data require a new bioinformatics focus on automatic methods for spectrum reprocessing and peptide/protein validation. Manual validation of results in such studies is not feasible and objective enough for quality relevant interpretation. The necessity for tools enabling an automatic quality control is, therefore, important to produce reliable and comparable data in such big consortia as the Human Proteome Organization Brain Proteome Project. Standards and well-defined processing pipelines are important for these consortia. We show a way for choosing the right database model, through collecting data, processing these with a decoy database and end up with a quality controlled protein list merged from several search engines, including a known false-positive rate.lld:pubmed
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pubmed-article:16927432pubmed:articleTitleAutomated reprocessing pipeline for searching heterogeneous mass spectrometric data of the HUPO Brain Proteome Project pilot phase.lld:pubmed
pubmed-article:16927432pubmed:affiliationMedizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany. Christian.Stephan@rub.delld:pubmed
pubmed-article:16927432pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16927432pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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