| pubmed-article:16914168 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16914168 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:16914168 | lifeskim:mentions | umls-concept:C0038556 | lld:lifeskim |
| pubmed-article:16914168 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:16914168 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16914168 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:16914168 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:16914168 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:16914168 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:16914168 | pubmed:issue | 22 | lld:pubmed |
| pubmed-article:16914168 | pubmed:dateCreated | 2006-10-23 | lld:pubmed |
| pubmed-article:16914168 | pubmed:abstractText | beta-Adrenoceptors (beta-ARs) mediate important physiological functions in salivary glands. Here we investigated the expression and function of beta-AR subtypes in rabbit submandibular gland (SMG). Both beta(1)- and beta(2)-ARs, but not beta(3)-AR, were strongly expressed in rabbit SMG. beta(1)-AR proteins were widely expressed in acinar and ductal cells whereas beta(2)-AR proteins were mainly detected in ductal cells. A [(3)H]-dihydroalprenolol binding assay revealed that beta-AR B(max) was 186+/-11.9 fmol/mg protein and K(d) was 2.71+/-0.23 nM. A competitive binding assay with CGP 20712A, a beta(1)-AR antagonist, indicated that the proportion of beta(1)-AR and beta(2)-AR was 71.9% and 28.1%, respectively. Gland perfusion with the beta-AR agonist isoproterenol induced a significant increase in saliva secretion which was abolished by pretreatment with the non-selective beta-AR antagonist propranolol. Pretreatment with beta(1)- or beta(2)-AR selective antagonists, CGP 20712A or ICI 118551, diminished isoproterenol-induced increase in saliva secretion by 71.2% and 28.8%, respectively. The expression of alpha-amylase mRNA was significantly stimulated by isoproterenol, which was eliminated by propranolol and CGP 20712A. Perfusion with isoproterenol decreased alpha-amylase protein storage in SMG and increased alpha-amylase activity in saliva. These alterations became less significant after pretreatment with propranolol and CGP 20712A. Our results suggest that both beta(1)- and beta(2)-ARs are expressed in rabbit SMG. beta(1)-AR is the predominant subtype and may play an important role in regulating saliva and alpha-amylase secretion. | lld:pubmed |
| pubmed-article:16914168 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16914168 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16914168 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16914168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16914168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16914168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16914168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16914168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16914168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16914168 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16914168 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:16914168 | pubmed:issn | 0024-3205 | lld:pubmed |
| pubmed-article:16914168 | pubmed:author | pubmed-author:ZhangYanY | lld:pubmed |
| pubmed-article:16914168 | pubmed:author | pubmed-author:YuGuang-YanGY | lld:pubmed |
| pubmed-article:16914168 | pubmed:author | pubmed-author:WuLi-LingLL | lld:pubmed |
| pubmed-article:16914168 | pubmed:author | pubmed-author:ZhangYou-YiYY | lld:pubmed |
| pubmed-article:16914168 | pubmed:author | pubmed-author:XiangBinB | lld:pubmed |
| pubmed-article:16914168 | pubmed:author | pubmed-author:LiYu-MingYM | lld:pubmed |
| pubmed-article:16914168 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16914168 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:16914168 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:16914168 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16914168 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16914168 | pubmed:pagination | 2091-8 | lld:pubmed |
| pubmed-article:16914168 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:16914168 | pubmed:meshHeading | pubmed-meshheading:16914168... | lld:pubmed |
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| pubmed-article:16914168 | pubmed:meshHeading | pubmed-meshheading:16914168... | lld:pubmed |
| pubmed-article:16914168 | pubmed:meshHeading | pubmed-meshheading:16914168... | lld:pubmed |
| pubmed-article:16914168 | pubmed:meshHeading | pubmed-meshheading:16914168... | lld:pubmed |
| pubmed-article:16914168 | pubmed:meshHeading | pubmed-meshheading:16914168... | lld:pubmed |
| pubmed-article:16914168 | pubmed:meshHeading | pubmed-meshheading:16914168... | lld:pubmed |
| pubmed-article:16914168 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16914168 | pubmed:articleTitle | Expression and functional analysis of beta-adrenoceptor subtypes in rabbit submandibular gland. | lld:pubmed |
| pubmed-article:16914168 | pubmed:affiliation | Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing 100083, China. | lld:pubmed |
| pubmed-article:16914168 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16914168 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
