| pubmed-article:16890197 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16890197 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
| pubmed-article:16890197 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:16890197 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:16890197 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:16890197 | lifeskim:mentions | umls-concept:C0456962 | lld:lifeskim |
| pubmed-article:16890197 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:16890197 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16890197 | pubmed:dateCreated | 2006-8-14 | lld:pubmed |
| pubmed-article:16890197 | pubmed:abstractText | A new, fast, and sensitive DNA hybridization assay platform based on microwave-accelerated metal-enhanced fluorescence (MAMEF) is presented. Thiolated oligonucleotide anchors were immobilized onto silver nanoparticles on a glass substrate. The hybridization of the complementary fluorescein-labeled DNA target with the surface-bound oligonucleotides was completed within 20 s upon heating with low-power microwaves. In addition, the signal is optically amplified, a consequence of close proximity of the fluorophore to the silvered substrate. In this proof-of-principle methodology, as low as 50 nM of a target DNA was detected, although we envisage far-lower detection limits. Control experiments, where the surface-bound oligonucleotide was omitted, were also performed to determine the extent of non-specific binding. In these studies a significantly reduced non-specific adsorption was found when using microwave heating near to silvered structures as compared to room temperature incubation. These findings suggest that MAMEF could be a most useful alternative to the DNA hybridization assays used today, especially with regard to substantially increasing both the assay rapidity and sensitivity. | lld:pubmed |
| pubmed-article:16890197 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16890197 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16890197 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16890197 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16890197 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16890197 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16890197 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16890197 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16890197 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16890197 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16890197 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:16890197 | pubmed:author | pubmed-author:GeddesChris... | lld:pubmed |
| pubmed-article:16890197 | pubmed:author | pubmed-author:AslanKadirK | lld:pubmed |
| pubmed-article:16890197 | pubmed:author | pubmed-author:MalynStuart... | lld:pubmed |
| pubmed-article:16890197 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16890197 | pubmed:day | 22 | lld:pubmed |
| pubmed-article:16890197 | pubmed:volume | 348 | lld:pubmed |
| pubmed-article:16890197 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16890197 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16890197 | pubmed:pagination | 612-7 | lld:pubmed |
| pubmed-article:16890197 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:16890197 | pubmed:meshHeading | pubmed-meshheading:16890197... | lld:pubmed |
| pubmed-article:16890197 | pubmed:meshHeading | pubmed-meshheading:16890197... | lld:pubmed |
| pubmed-article:16890197 | pubmed:meshHeading | pubmed-meshheading:16890197... | lld:pubmed |
| pubmed-article:16890197 | pubmed:meshHeading | pubmed-meshheading:16890197... | lld:pubmed |
| pubmed-article:16890197 | pubmed:meshHeading | pubmed-meshheading:16890197... | lld:pubmed |
| pubmed-article:16890197 | pubmed:meshHeading | pubmed-meshheading:16890197... | lld:pubmed |
| pubmed-article:16890197 | pubmed:meshHeading | pubmed-meshheading:16890197... | lld:pubmed |
| pubmed-article:16890197 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16890197 | pubmed:articleTitle | Fast and sensitive DNA hybridization assays using microwave-accelerated metal-enhanced fluorescence. | lld:pubmed |
| pubmed-article:16890197 | pubmed:affiliation | Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard St, Baltimore, MD 21201, USA. | lld:pubmed |
| pubmed-article:16890197 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16890197 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16890197 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
