| pubmed-article:16884969 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16884969 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:16884969 | lifeskim:mentions | umls-concept:C0170657 | lld:lifeskim |
| pubmed-article:16884969 | lifeskim:mentions | umls-concept:C0597360 | lld:lifeskim |
| pubmed-article:16884969 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16884969 | pubmed:dateCreated | 2006-12-4 | lld:pubmed |
| pubmed-article:16884969 | pubmed:abstractText | Neurotransmitter regulation of bone metabolism has been a subject of increasing interest and investigation. We reported previously that osteoblastic cells express a functional serotonin (5-HT) signal transduction system, with mechanisms for responding to and regulating uptake of 5-HT. The clonal murine osteocytic cell line, MLO-Y4, demonstrates expression of the serotonin transporter (5-HTT), and the 5-HT1A, and 5-HT2A receptors by real-time RT-PCR and immunoblot analysis. Immunohistochemistry using antibodies for the 5-HTT, and the 5-HT1A and 5-HT2A receptors reveals expression of all three proteins in both osteoblasts and osteocytes in rat tibia. 5-HTT binding sites were demonstrated in the MLO-Y4 cells with nanomolar affinity for the stable cocaine analog [125I]RTI-55. Imipramine and fluoxetine, antagonists with specificity for 5-HTT, show the highest potency to antagonize [125I]RTI-55 binding in the MLO-Y4 cells. GBR-12935, a relatively selective dopamine transporter antagonist, had a much lower potency, as did desipramine, a selective norepinephrine transporter antagonist. The maximal [3H]5-HT uptake rate in MLO-Y4 cells was 2.85 pmol/15 min/well, with a Km value of 290 nM. Imipramine and fluoxetine inhibited specific [3H]5-HT uptake with IC50 values in the nanomolar range. 5-HT rapidly stimulated PGE2 release from MLO-Y4 cells; the EC50 for 5-HT was 0.1 microM, with a 3-fold increase seen at 60 min. The rate-limiting enzyme for serotonin synthesis, tryptophan hydroxylase, is expressed in MLO-Y4 cells as well as osteoblastic MC3T3-E1 cells. Thus, osteocytes, as well as osteoblasts, are capable of 5-HT synthesis, and express functional receptor and transporter components of the 5-HT signal transduction system. | lld:pubmed |
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| pubmed-article:16884969 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:16884969 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16884969 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16884969 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:16884969 | pubmed:issn | 8756-3282 | lld:pubmed |
| pubmed-article:16884969 | pubmed:author | pubmed-author:HashimotoJJ | lld:pubmed |
| pubmed-article:16884969 | pubmed:author | pubmed-author:ChenuCC | lld:pubmed |
| pubmed-article:16884969 | pubmed:author | pubmed-author:BliziotesMM | lld:pubmed |
| pubmed-article:16884969 | pubmed:author | pubmed-author:EshlemanAA | lld:pubmed |
| pubmed-article:16884969 | pubmed:author | pubmed-author:Burt-PichatBB | lld:pubmed |
| pubmed-article:16884969 | pubmed:author | pubmed-author:WirenKK | lld:pubmed |
| pubmed-article:16884969 | pubmed:author | pubmed-author:ZhangX-WXW | lld:pubmed |
| pubmed-article:16884969 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16884969 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:16884969 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16884969 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16884969 | pubmed:pagination | 1313-21 | lld:pubmed |
| pubmed-article:16884969 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:16884969 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16884969 | pubmed:articleTitle | Serotonin transporter and receptor expression in osteocytic MLO-Y4 cells. | lld:pubmed |
| pubmed-article:16884969 | pubmed:affiliation | Portland VA Medical Center, Portland, OR 97239, USA. bliziote@ohsu.edu | lld:pubmed |
| pubmed-article:16884969 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16884969 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:16884969 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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