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pubmed-article:16881513pubmed:abstractTextThis chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.lld:pubmed
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pubmed-article:16881513pubmed:dateRevised2009-11-19lld:pubmed
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pubmed-article:16881513pubmed:articleTitleManipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.lld:pubmed
pubmed-article:16881513pubmed:affiliationLaboratory of Molecular Embryology, The Rockefeller University, New York, NY, USA.lld:pubmed
pubmed-article:16881513pubmed:publicationTypeJournal Articlelld:pubmed