| pubmed-article:16879746 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16879746 | lifeskim:mentions | umls-concept:C0041455 | lld:lifeskim |
| pubmed-article:16879746 | lifeskim:mentions | umls-concept:C0011644 | lld:lifeskim |
| pubmed-article:16879746 | lifeskim:mentions | umls-concept:C0301872 | lld:lifeskim |
| pubmed-article:16879746 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:16879746 | pubmed:dateCreated | 2007-1-16 | lld:pubmed |
| pubmed-article:16879746 | pubmed:abstractText | This study was conducted to examine the frequency, phenotype, and functional profile of T lymphocytes that proliferate in response to type I collagen (CI) in patients with scleroderma (SSc). Peripheral blood mononuclear cells (PBMCs) from SSc patients, healthy controls, and rheumatoid arthritis disease controls were labeled with carboxy-fluorescein diacetate, succinimidyl ester (CFSE), cultured with or without antigen (bovine CI) for 14 days, and analysed by flow cytometry. Surface markers of proliferating cells were identified by multi-color flow cytometry. T-cell lines were derived after sorting for proliferating T cells (CFSElow). Cytokine expression in CI-responsive T cells was detected by intracellular staining/flow cytometry and by multiplex cytokine bead assay (Bio-Plex). A T-cell proliferative response to CI was detected in 8 of 25 (32%) SSc patients, but was infrequent in healthy or disease controls (3.6%; p = 0.009). The proliferating T cells expressed a CD4+, activated (CD25+), memory (CD45RO+) phenotype. Proliferation to CI did not correlate with disease duration or extent of skin involvement. T-cell lines were generated using in vitro CI stimulation to study the functional profile of these cells. Following activation of CI-reactive T cells, we detected intracellular interferon (IFN)-gamma but not interleukin (IL)-4 by flow cytometry. Supernatants from the T-cell lines generated in vitro contained IL-2, IFN-gamma, GM-CSF (granulocyte macrophage-colony-stimulating factor), and tumour necrosis factor-alpha, but little or no IL-4 and IL-10, suggesting that CI-responsive T cells express a predominantly Th1 cytokine pattern. In conclusion, circulating memory CD4 T cells that proliferate to CI are present in a subset of patients with SSc, but are infrequent in healthy or disease controls. | lld:pubmed |
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| pubmed-article:16879746 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:16879746 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16879746 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16879746 | pubmed:issn | 1478-6362 | lld:pubmed |
| pubmed-article:16879746 | pubmed:author | pubmed-author:KangAndrew... | lld:pubmed |
| pubmed-article:16879746 | pubmed:author | pubmed-author:WarringtonKen... | lld:pubmed |
| pubmed-article:16879746 | pubmed:author | pubmed-author:NairUshaU | lld:pubmed |
| pubmed-article:16879746 | pubmed:author | pubmed-author:Postlethwaite... | lld:pubmed |
| pubmed-article:16879746 | pubmed:author | pubmed-author:CarboneLaura... | lld:pubmed |
| pubmed-article:16879746 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:16879746 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:16879746 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16879746 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16879746 | pubmed:pagination | R136 | lld:pubmed |
| pubmed-article:16879746 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:16879746 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16879746 | pubmed:articleTitle | Characterisation of the immune response to type I collagen in scleroderma. | lld:pubmed |
| pubmed-article:16879746 | pubmed:affiliation | Department of Medicine, Division of Connective Tissue Diseases, 956 Court Avenue, Room G326, Memphis, TN 38163, USA. warrington.kenneth@mayo.edu | lld:pubmed |
| pubmed-article:16879746 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16879746 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:16879746 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16879746 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
