| pubmed-article:16837047 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0016163 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0021753 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0386482 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0233820 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C1999230 | lld:lifeskim |
| pubmed-article:16837047 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:16837047 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16837047 | pubmed:dateCreated | 2006-9-26 | lld:pubmed |
| pubmed-article:16837047 | pubmed:abstractText | Mammalian interleukin-1beta (IL-1beta) is produced as a biologically inactive precursor molecule, which is proteolytically cleaved to an active form by IL-1beta-converting enzyme (ICE) after the activation of P2X(7) receptor by extracellular ATP. The mechanism of IL-1beta release in non-mammalian vertebrates is largely unknown, although most of the IL-1beta gene sequences lack a conserved ICE recognition site. Here we have cloned the P2X(7) receptor from the bony fish seabream and compared agonist and antagonist profiles at this and other non-mammalian P2X(7) receptors expressed in HEK cells, as well in seabream SAF-1 cells expressing endogenous P2X(7) receptors. We used this information to further investigate the mechanisms of IL-1beta release induced by mammalian and fish P2X(7) receptors. Despite phosphatidylserine externalization and cell permeabilization in seabream leukocytes after the addition of high BzATP concentrations, IL-1beta remained unprocessed within the cell. However, activation of rat P2X(7) receptors ectopically expressed in HEK293 together with human ICE led to the specific secretion of unprocessed seabream IL-1beta. In contrast, neither seabream nor zebrafish P2X(7) receptors induced the secretion of mammalian or fish IL-1beta when expressed in HEK293, while a chimeric receptor harboring the ATP-binding domain of seabream P2X(7) and the intracellular region of its rat counterpart did so. These findings indicate that P2X(7) receptor-mediated activation of ICE and release of IL-1beta result from different downstream signaling pathways and suggest that although the mechanisms involved in IL-1beta secretion are conserved throughout evolution, distinct inflammatory signals have been selected for the secretion of this cytokine in different vertebrates. | lld:pubmed |
| pubmed-article:16837047 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16837047 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16837047 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16837047 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16837047 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16837047 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16837047 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16837047 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16837047 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16837047 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16837047 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16837047 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:16837047 | pubmed:issn | 0161-5890 | lld:pubmed |
| pubmed-article:16837047 | pubmed:author | pubmed-author:MeseguerJoséJ | lld:pubmed |
| pubmed-article:16837047 | pubmed:author | pubmed-author:MuleroVictori... | lld:pubmed |
| pubmed-article:16837047 | pubmed:author | pubmed-author:SurprenantAnn... | lld:pubmed |
| pubmed-article:16837047 | pubmed:author | pubmed-author:YoungMark TMT | lld:pubmed |
| pubmed-article:16837047 | pubmed:author | pubmed-author:López-Castejó... | lld:pubmed |
| pubmed-article:16837047 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16837047 | pubmed:volume | 44 | lld:pubmed |
| pubmed-article:16837047 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16837047 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16837047 | pubmed:pagination | 1286-99 | lld:pubmed |
| pubmed-article:16837047 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:meshHeading | pubmed-meshheading:16837047... | lld:pubmed |
| pubmed-article:16837047 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:16837047 | pubmed:articleTitle | Characterization of ATP-gated P2X7 receptors in fish provides new insights into the mechanism of release of the leaderless cytokine interleukin-1 beta. | lld:pubmed |
| pubmed-article:16837047 | pubmed:affiliation | Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain. | lld:pubmed |
| pubmed-article:16837047 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16837047 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:16837047 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:387298 | entrezgene:pubmed | pubmed-article:16837047 | lld:entrezgene |
