| pubmed-article:168222 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:168222 | lifeskim:mentions | umls-concept:C0237868 | lld:lifeskim |
| pubmed-article:168222 | lifeskim:mentions | umls-concept:C0001128 | lld:lifeskim |
| pubmed-article:168222 | lifeskim:mentions | umls-concept:C0036679 | lld:lifeskim |
| pubmed-article:168222 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:168222 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:168222 | pubmed:dateCreated | 1975-11-5 | lld:pubmed |
| pubmed-article:168222 | pubmed:abstractText | A method for the separation of all phenylthiohydantoin (PTH)-amino acids except PTH-arginine and PTH-histidine by high-pressure liquid chromatography on a silica column is described. Elution is performed with a concave solvent gradient from hexane-methanol-propanol (3980:9:11) to methanol-propanol (9:11). A complete run is achieved in 40 min with a pressure drop of 1000 p.s.i. over the 250 mm X 2.1 mm column. Eluted peaks of 2-5 nmole are easily detected by their ultraviolet absorption at 254 nm. This method is superior to existing gas-liquid and thin-layer chromatographic techniques since all PTH-amino acids except PTH-arginine and PTH-histidine may be both separated and quantitated in a single run of 40 min. The use of the technique in conjunction with an automated peptide sequence analyser is illustrated. | lld:pubmed |
| pubmed-article:168222 | pubmed:language | eng | lld:pubmed |
| pubmed-article:168222 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:168222 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:168222 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:168222 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:168222 | pubmed:author | pubmed-author:MatthewsE WEW | lld:pubmed |
| pubmed-article:168222 | pubmed:author | pubmed-author:ByfieldP GPG | lld:pubmed |
| pubmed-article:168222 | pubmed:author | pubmed-author:MacIntyreII | lld:pubmed |
| pubmed-article:168222 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:168222 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:168222 | pubmed:volume | 110 | lld:pubmed |
| pubmed-article:168222 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:168222 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:168222 | pubmed:pagination | 369-73 | lld:pubmed |
| pubmed-article:168222 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:168222 | pubmed:meshHeading | pubmed-meshheading:168222-P... | lld:pubmed |
| pubmed-article:168222 | pubmed:meshHeading | pubmed-meshheading:168222-A... | lld:pubmed |
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| pubmed-article:168222 | pubmed:meshHeading | pubmed-meshheading:168222-C... | lld:pubmed |
| pubmed-article:168222 | pubmed:meshHeading | pubmed-meshheading:168222-T... | lld:pubmed |
| pubmed-article:168222 | pubmed:year | 1975 | lld:pubmed |
| pubmed-article:168222 | pubmed:articleTitle | Separation of phenylthiohydantoin-amino acids by high-pressure liquid chromatography. | lld:pubmed |
| pubmed-article:168222 | pubmed:publicationType | Journal Article | lld:pubmed |
