| pubmed-article:16806786 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16806786 | lifeskim:mentions | umls-concept:C0007592 | lld:lifeskim |
| pubmed-article:16806786 | lifeskim:mentions | umls-concept:C0008562 | lld:lifeskim |
| pubmed-article:16806786 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:16806786 | lifeskim:mentions | umls-concept:C1720880 | lld:lifeskim |
| pubmed-article:16806786 | lifeskim:mentions | umls-concept:C2699488 | lld:lifeskim |
| pubmed-article:16806786 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16806786 | pubmed:dateCreated | 2006-8-28 | lld:pubmed |
| pubmed-article:16806786 | pubmed:abstractText | A stereospecific HPLC methodology has been developed for the diastereoisomeric resolution of a mononucleotide prodrug in cell extracts. This method involves the use of solid phase extraction on a C18 cartridge. Diastereoisomers and internal standard resolutions were performed on a cellulose based chiral column (Chiralcel OD-H) used in the normal phase mode. The method was validated in terms of specificity, recovery, linearity (diasteroisomers mixture concentration: 3-60 micromol L(-1)), precision and accuracy and detection limit (1.67 and 1.33 micromol L(-1) for first and second eluted diastereoisomer). This method was applied to the determination of the apparent rate constants of disappearance and half-lives of each stereoisomers. This permits to conclude to the stereoselectivity of the enzymatic activity involved in the decomposition pathway of 2. | lld:pubmed |
| pubmed-article:16806786 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16806786 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16806786 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16806786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16806786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16806786 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16806786 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16806786 | pubmed:issn | 0731-7085 | lld:pubmed |
| pubmed-article:16806786 | pubmed:author | pubmed-author:FowlerJ MJM | lld:pubmed |
| pubmed-article:16806786 | pubmed:author | pubmed-author:PazJ AJA | lld:pubmed |
| pubmed-article:16806786 | pubmed:author | pubmed-author:VaccherCC | lld:pubmed |
| pubmed-article:16806786 | pubmed:author | pubmed-author:LefebvreII | lld:pubmed |
| pubmed-article:16806786 | pubmed:author | pubmed-author:GoossensJ FJF | lld:pubmed |
| pubmed-article:16806786 | pubmed:author | pubmed-author:BonteJ PJP | lld:pubmed |
| pubmed-article:16806786 | pubmed:author | pubmed-author:PerigaudCC | lld:pubmed |
| pubmed-article:16806786 | pubmed:author | pubmed-author:VillardA-LAL | lld:pubmed |
| pubmed-article:16806786 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16806786 | pubmed:day | 18 | lld:pubmed |
| pubmed-article:16806786 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:16806786 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16806786 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16806786 | pubmed:pagination | 245-52 | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:meshHeading | pubmed-meshheading:16806786... | lld:pubmed |
| pubmed-article:16806786 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16806786 | pubmed:articleTitle | Diastereoisomeric resolution of a pronucleotide using solid phase extraction and high performance liquid chromatography: application to a stereoselective decomposition kinetic in cell extracts. | lld:pubmed |
| pubmed-article:16806786 | pubmed:affiliation | Laboratoire de Chimie Analytique, EA 4034, Faculté des Sciences Pharmaceutiques et Biologiques, Université de LILLE 2, BP 83, 3 rue du Pr. Laguesse, 59006 Lille Cedex, France. | lld:pubmed |
| pubmed-article:16806786 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16806786 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
