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pubmed-article:1680604pubmed:abstractTextThe presence of histamine H1-receptors in the bovine retinal blood vessels was studied with a [3H]mepyramine binding assay. The membranes of purified vessels obtained from bovine retinas showed specific [3H]mepyramine binding sites with a dissociation constant (KD) of 2.78 +/- 0.32 nM. This was similar to values obtained from the retinal neuronal fractions. The binding capacity (Bmax) was 53.8 +/- 1.7 fmol/mg protein, which was about a half that of the retinal neuronal fractions (108.9 +/- 3.1 fmol/mg protein). Some H1-antagonists proved to be potent competitors for [3H]mepyramine binding sites in bovine retinal blood vessels. These results indicate that histamine H1-receptors exist in the retinal blood vessels which may be involved in the physiological and the pathological responses of blood circulation in retinas.lld:pubmed
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pubmed-article:1680604pubmed:articleTitle[3H]mepyramine binding sites, histamine H1-receptors, in bovine retinal blood vessels.lld:pubmed
pubmed-article:1680604pubmed:affiliationDepartment of Ophthalmology, Osaka University Hospital, Japan.lld:pubmed
pubmed-article:1680604pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1680604pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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