| pubmed-article:16788968 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C0262950 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C1257975 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C2755105 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C2828366 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C1705851 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C1555707 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:16788968 | lifeskim:mentions | umls-concept:C2752151 | lld:lifeskim |
| pubmed-article:16788968 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:16788968 | pubmed:dateCreated | 2006-10-24 | lld:pubmed |
| pubmed-article:16788968 | pubmed:abstractText | Since its osteoinductive capacity has been established, demineralized bone matrix is considered a suitable alternative to bone autograft in the healing of osseous defects. The mechanisms of bone formation induction are still not fully understood. In this study we assessed the effects of a dispersion of bovine bone extract COLLOSS (BPE) with regard to proliferation and differentiation of a human mesenchymal stem cell line overexpressing human telomerase reverse transcriptase (hMSC-TERT). Proliferation rate was determined by (3)H-thymidine incorporation. The differentiation of hMSC-TERT cells to osteoblastic cells was assessed by means of measuring alkaline phosphatase activity and collagen synthesis in vitro. Both undifferentiated and osteoblast-differentiated hMSC-TERT cells were investigated for response to BPE. The metabolic responses to BPE were compared to unstimulated cells and cells stimulated with bovine collagen (COL). Undifferentiated hMSC-TERT cells responded to BPE with increased proliferation and decreased alkaline phosphatase activity. Osteoblastic differentiated hMSC-TERT cells had a diminished proliferative response and an increased alkaline phosphatase activity and collagen synthesis. Our study demonstrated significant metabolic effects of BPE on hMSC-TERT cells, which were highly dependant on the differentiated state of the cells. | lld:pubmed |
| pubmed-article:16788968 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16788968 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16788968 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16788968 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16788968 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16788968 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16788968 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16788968 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:16788968 | pubmed:issn | 1549-3296 | lld:pubmed |
| pubmed-article:16788968 | pubmed:author | pubmed-author:LindMartinM | lld:pubmed |
| pubmed-article:16788968 | pubmed:author | pubmed-author:LiHaishengH | lld:pubmed |
| pubmed-article:16788968 | pubmed:author | pubmed-author:KassemMoustap... | lld:pubmed |
| pubmed-article:16788968 | pubmed:author | pubmed-author:KrauseAndreaA | lld:pubmed |
| pubmed-article:16788968 | pubmed:author | pubmed-author:BaatrupAnette... | lld:pubmed |
| pubmed-article:16788968 | pubmed:author | pubmed-author:WooCharlotteC | lld:pubmed |
| pubmed-article:16788968 | pubmed:author | pubmed-author:BungerCodyC | lld:pubmed |
| pubmed-article:16788968 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16788968 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:16788968 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:16788968 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16788968 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16788968 | pubmed:pagination | 552-6 | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:meshHeading | pubmed-meshheading:16788968... | lld:pubmed |
| pubmed-article:16788968 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16788968 | pubmed:articleTitle | Effects of bone protein extract on human mesenchymal stem cells proliferation and differentiation. | lld:pubmed |
| pubmed-article:16788968 | pubmed:affiliation | Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, DK-8000 Aarhus C, Denmark. | lld:pubmed |
| pubmed-article:16788968 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16788968 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
