| pubmed-article:16782188 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16782188 | lifeskim:mentions | umls-concept:C0007577 | lld:lifeskim |
| pubmed-article:16782188 | lifeskim:mentions | umls-concept:C2698172 | lld:lifeskim |
| pubmed-article:16782188 | lifeskim:mentions | umls-concept:C1708533 | lld:lifeskim |
| pubmed-article:16782188 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:16782188 | pubmed:issue | 29 | lld:pubmed |
| pubmed-article:16782188 | pubmed:dateCreated | 2006-7-3 | lld:pubmed |
| pubmed-article:16782188 | pubmed:abstractText | Bio-functionalized thermoresponsive culture interfaces co-immobilized with cell adhesive peptide, RGDS, and cell growth factor, insulin (INS), are investigated to promote initial cell adhesion and cell growth for further cell sheet engineering applications. These bio-functionalized interfaces were prepared by electron beam-induced copolymerization of N-isopropylacrylamide (IPAAm) with its carboxyl-derivatized analog, 2-carboxyisopropylacrylamide (CIPAAm), and grafting onto tissue culture polystyrene dishes, followed by immobilization of RGDS and/or INS to CIPAAm carboxyls. Adhesion and proliferation of bovine carotid artery endothelial cells (ECs) were examined on the RGDS-INS co-immobilized thermoresponsive interfaces. Immobilized RGDS facilitated initial EC adhesion on the surfaces and INS modification was demonstrated to induce EC proliferation, respectively. More pronounced EC growth was indicated by co-immobilization of appropriate amount of RGDS and INS. This may be due to synergistic effect of direct co-stimulation of adhered ECs by surface-immobilized RGDS and INS molecules. ECs grown on the RGDS-INS co-immobilized thermoresponsive interfaces can also be recovered spontaneously as viable tissue monolayers by solely reducing culture temperature. RGDS-INS co-immobilized thermoresponsive interfaces strongly supported initial EC adhesion and growth than unmodified thermoresponsive surfaces even under serum-free culture. Addition of soluble growth factors to serum-free culture medium effectively induced EC proliferation to confluency. Co-immobilization of cell adhesion peptides and growth factors on thermoresponsive surfaces should be effective for rapid preparation of intact cell sheets and their utilization to regenerative medicine. | lld:pubmed |
| pubmed-article:16782188 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16782188 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16782188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16782188 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16782188 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:16782188 | pubmed:issn | 0142-9612 | lld:pubmed |
| pubmed-article:16782188 | pubmed:author | pubmed-author:KikuchiAkihik... | lld:pubmed |
| pubmed-article:16782188 | pubmed:author | pubmed-author:OkanoTeruoT | lld:pubmed |
| pubmed-article:16782188 | pubmed:author | pubmed-author:YamatoMasayuk... | lld:pubmed |
| pubmed-article:16782188 | pubmed:author | pubmed-author:HatakeyamaHid... | lld:pubmed |
| pubmed-article:16782188 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16782188 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:16782188 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16782188 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16782188 | pubmed:pagination | 5069-78 | lld:pubmed |
| pubmed-article:16782188 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:meshHeading | pubmed-meshheading:16782188... | lld:pubmed |
| pubmed-article:16782188 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16782188 | pubmed:articleTitle | Bio-functionalized thermoresponsive interfaces facilitating cell adhesion and proliferation. | lld:pubmed |
| pubmed-article:16782188 | pubmed:affiliation | Institute of Advanced Biomedical Engineering and Science, Center of Excellence (COE) Program for the 21st Century, Tokyo Women's Medical University, Tokyo, Japan. | lld:pubmed |
| pubmed-article:16782188 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16782188 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16782188 | lld:pubmed |
