| pubmed-article:16777066 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C0023621 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C0205148 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C0870432 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C1705417 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C1555465 | lld:lifeskim |
| pubmed-article:16777066 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:16777066 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:16777066 | pubmed:dateCreated | 2006-6-26 | lld:pubmed |
| pubmed-article:16777066 | pubmed:abstractText | Lymphocytes from eight individuals out of 60 healthy donors, whose plasmas showed relatively higher antibody titer for a target antigen of death receptor 5 (DR5), were selected for the source of antibody genes to construct so called an anti-DR5 pseudo-immune human single-chain fragment variable (scFv) library on the yeast cell surface (approximately 2x10(6) diversity). Compared with a large nonimmune human scFv library (approximately 1x10(9) diversity), the repertoire of the pseudo-immune scFv library was significantly biased toward the target antigen, which facilitated rapid enrichments of the target-specific high affinity scFvs during selections by fluorescence activated cell sortings. Isolated scFvs, HW5 and HW6, from the pseudo-immune library showed much higher specificity and affinity for the targeted antigen than those from the nonimmune library. Our results suggest that a pseudo-immune antibody library is very efficient to isolate target-specific high affinity antibody from a relatively small sized library. | lld:pubmed |
| pubmed-article:16777066 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16777066 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16777066 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16777066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16777066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16777066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16777066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16777066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16777066 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16777066 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16777066 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:16777066 | pubmed:author | pubmed-author:KimYong-SungY... | lld:pubmed |
| pubmed-article:16777066 | pubmed:author | pubmed-author:KimJeong-SunJ... | lld:pubmed |
| pubmed-article:16777066 | pubmed:author | pubmed-author:KwonMyung-Hee... | lld:pubmed |
| pubmed-article:16777066 | pubmed:author | pubmed-author:LeeSeung-Hyun... | lld:pubmed |
| pubmed-article:16777066 | pubmed:author | pubmed-author:LeeHae-WonHW | lld:pubmed |
| pubmed-article:16777066 | pubmed:author | pubmed-author:ParkKyung-Jin... | lld:pubmed |
| pubmed-article:16777066 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16777066 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:16777066 | pubmed:volume | 346 | lld:pubmed |
| pubmed-article:16777066 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16777066 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16777066 | pubmed:pagination | 896-903 | lld:pubmed |
| pubmed-article:16777066 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16777066 | pubmed:meshHeading | pubmed-meshheading:16777066... | lld:pubmed |
| pubmed-article:16777066 | pubmed:meshHeading | pubmed-meshheading:16777066... | lld:pubmed |
| pubmed-article:16777066 | pubmed:meshHeading | pubmed-meshheading:16777066... | lld:pubmed |
| pubmed-article:16777066 | pubmed:meshHeading | pubmed-meshheading:16777066... | lld:pubmed |
| pubmed-article:16777066 | pubmed:meshHeading | pubmed-meshheading:16777066... | lld:pubmed |
| pubmed-article:16777066 | pubmed:meshHeading | pubmed-meshheading:16777066... | lld:pubmed |
| pubmed-article:16777066 | pubmed:meshHeading | pubmed-meshheading:16777066... | lld:pubmed |
| pubmed-article:16777066 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16777066 | pubmed:articleTitle | Construction and characterization of a pseudo-immune human antibody library using yeast surface display. | lld:pubmed |
| pubmed-article:16777066 | pubmed:affiliation | Department of Molecular Science and Technology, Ajou University, San 5, Woncheon-dong, Yeongtong-gu, Suwon 443-749, Republic of Korea. | lld:pubmed |
| pubmed-article:16777066 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16777066 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16777066 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16777066 | lld:pubmed |
