| pubmed-article:16763664 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16763664 | lifeskim:mentions | umls-concept:C0017296 | lld:lifeskim |
| pubmed-article:16763664 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:16763664 | lifeskim:mentions | umls-concept:C0205373 | lld:lifeskim |
| pubmed-article:16763664 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:16763664 | pubmed:dateCreated | 2006-10-17 | lld:pubmed |
| pubmed-article:16763664 | pubmed:abstractText | We recently reported a novel coupling strategy involving salicylhydroxamic acid and phenyl(di)boronic acid molecules to attach the CNGRC peptide to PEI/DNA for CD13 targeting in tumors. Here, we doubly coupled Simian Virus (SV) 40 peptide-(nuclear localization signal)) and oligonucleotide-based (DNA nuclear targeting signal) nuclear signals to the same vector using peptide nucleic acid chemistry. This vector, CNGRC/PEG/PEI/DNA-betagal/NLS/DNTS, was predominantly localized in the cell nucleus, yielding about 200-fold higher betagal gene expression in vitro, more than 20-fold increase in tumor-specific gene delivery, and a robust betagal gene expression as demonstrated in stained tumor sections. For gene therapy purposes, we further engineered a similar targeting polyplex, CNGRC/PEG/PEI/DNA-p53/NLS/DNTS, with EBV-based episomal vector for sustained p53 gene expression. A distribution of vector DNA and apoptosis in p53-containing tumors was observed, yielding a significant tumor regression and 95% animal survival after 60 days. This multicomponent vector also co-targeted tumor and tumor-associated endothelial cells but not normal cells, and had more efficient therapeutic index than each vector administered as a single modality. The use of an efficient coupling strategy without compromising the vector's integrity for DNA condensation and endosomal escape; nuclear import; tumor-specific and persistent p53 gene expression clearly provides a basis for developing a single combinatorial approach for non-viral gene therapy. | lld:pubmed |
| pubmed-article:16763664 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16763664 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16763664 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16763664 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16763664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16763664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16763664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16763664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16763664 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16763664 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:16763664 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:16763664 | pubmed:author | pubmed-author:CristianoR... | lld:pubmed |
| pubmed-article:16763664 | pubmed:author | pubmed-author:MoffattSS | lld:pubmed |
| pubmed-article:16763664 | pubmed:author | pubmed-author:WiehleSS | lld:pubmed |
| pubmed-article:16763664 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16763664 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:16763664 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16763664 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16763664 | pubmed:pagination | 1512-23 | lld:pubmed |
| pubmed-article:16763664 | pubmed:dateRevised | 2007-12-3 | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:meshHeading | pubmed-meshheading:16763664... | lld:pubmed |
| pubmed-article:16763664 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16763664 | pubmed:articleTitle | A multifunctional PEI-based cationic polyplex for enhanced systemic p53-mediated gene therapy. | lld:pubmed |
| pubmed-article:16763664 | pubmed:affiliation | Department of Genitourinary Medical Oncology, University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA. stanley-moffatt@excite.com | lld:pubmed |
| pubmed-article:16763664 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16763664 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16763664 | lld:pubmed |
