pubmed-article:16723505 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C0037864 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C0040648 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C0597298 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C0678723 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C0332298 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C1707719 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C1523987 | lld:lifeskim |
pubmed-article:16723505 | lifeskim:mentions | umls-concept:C0458003 | lld:lifeskim |
pubmed-article:16723505 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:16723505 | pubmed:dateCreated | 2006-8-24 | lld:pubmed |
pubmed-article:16723505 | pubmed:abstractText | Spermatogeniccells elaborate a highly specialized differentiation program that is mediated in part by germ cell-enriched transcription factors. This includes a novel member of the sterol response element-binding factor family, SREBF2_v1/SREBP2gc. Somatic SREBFs are predominantly synthesized as precursor proteins and are critical regulators of cholesterol and fatty acid synthesis. In contrast, SREBF2_v1 bypasses the precursor pathway and has been directly implicated in spermatogenic cell-specific gene expression. During spermatogenesis, SREBF2 precursor transcripts predominate in premeiotic stages, while SREBF2_v1 is highly upregulated specifically in pachytene spermatocytes and round spermatids. In the present study, we demonstrate thatSrebf2_v1mRNAs are present in the testis of several mammalian species, including humans. The basis for the stage-dependent transition in SREBF2 isoforms was also investigated. A 3' rapid amplification of cDNA ends (RACE)-PCR analysis of the rat and human revealed thatSrebf2_v1transcripts are generated by alternative pre-mRNA cleavage/polyadenylation. This involves the use of an intronic, A(A/U)UAAA-independent poly(A) signal within intron 7 of theSrebf2gene. Developmentally regulated competition between germ cell factors that control RNA splicing and pre-mRNA cleavage/polyadenylation may underlie this process. These results define an important role for alternative polyadenylation in male germ cell gene expression and development by controlling a stage-dependent switch in transcription factor structure and function during spermatogenesis. TheSrebf2gene thus provides a useful model to explore the role of alternative polyadenylation in regulating stage-dependent functions of important protein regulators in spermatogenic cells. | lld:pubmed |
pubmed-article:16723505 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16723505 | pubmed:language | eng | lld:pubmed |
pubmed-article:16723505 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16723505 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:16723505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16723505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16723505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16723505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16723505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16723505 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:16723505 | pubmed:month | Sep | lld:pubmed |
pubmed-article:16723505 | pubmed:issn | 0006-3363 | lld:pubmed |
pubmed-article:16723505 | pubmed:author | pubmed-author:MilletteClark... | lld:pubmed |
pubmed-article:16723505 | pubmed:author | pubmed-author:WangHangH | lld:pubmed |
pubmed-article:16723505 | pubmed:author | pubmed-author:KilpatrickDan... | lld:pubmed |
pubmed-article:16723505 | pubmed:author | pubmed-author:SartiniBecky... | lld:pubmed |
pubmed-article:16723505 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:16723505 | pubmed:volume | 75 | lld:pubmed |
pubmed-article:16723505 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:16723505 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:16723505 | pubmed:pagination | 318-23 | lld:pubmed |
pubmed-article:16723505 | pubmed:dateRevised | 2007-12-3 | lld:pubmed |
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pubmed-article:16723505 | pubmed:year | 2006 | lld:pubmed |
pubmed-article:16723505 | pubmed:articleTitle | A developmental switch in transcription factor isoforms during spermatogenesis controlled by alternative messenger RNA 3'-end formation. | lld:pubmed |
pubmed-article:16723505 | pubmed:affiliation | Department of Molecular and Cellular Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA. | lld:pubmed |
pubmed-article:16723505 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:16723505 | pubmed:publicationType | In Vitro | lld:pubmed |
pubmed-article:16723505 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
entrez-gene:300095 | entrezgene:pubmed | pubmed-article:16723505 | lld:entrezgene |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16723505 | lld:pubmed |