pubmed-article:167189 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:167189 | lifeskim:mentions | umls-concept:C1623048 | lld:lifeskim |
pubmed-article:167189 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
pubmed-article:167189 | lifeskim:mentions | umls-concept:C1623415 | lld:lifeskim |
pubmed-article:167189 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
pubmed-article:167189 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
pubmed-article:167189 | lifeskim:mentions | umls-concept:C1556066 | lld:lifeskim |
pubmed-article:167189 | lifeskim:mentions | umls-concept:C1619636 | lld:lifeskim |
pubmed-article:167189 | lifeskim:mentions | umls-concept:C1514873 | lld:lifeskim |
pubmed-article:167189 | pubmed:issue | 6 | lld:pubmed |
pubmed-article:167189 | pubmed:dateCreated | 1975-9-29 | lld:pubmed |
pubmed-article:167189 | pubmed:abstractText | Vesicular stomatitis virions, Indiana serotype, were solubilized with high salt solubilizer and separated by ultracentrifugation into a supernatant fraction containing L, G, NS, and M proteins and pellet fraction containing the RNA complexed with N protein. NS protein was purified from the supernatnat fluid by sequential chromatography on phosphocellulose and diethylaminoethyl cellulose columns. The purified NS protein was assayed in a standard transcription system in combination with purified L protein and purified template (pellet fraction) prepared by renografin or CsCl banding. Results of the polymerase assays indicate that both L and NS proteins are required to reconstitute transcription activity with a highly purified template composed of only RNA and N protein. The NS protein polymerase activity is destroyed by trypsin but withstands 90 C temperatures for 10 min. Cytoplasmic NS protein can substitute for virion NS protein in the in vitro transcription assay. | lld:pubmed |
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pubmed-article:167189 | pubmed:language | eng | lld:pubmed |
pubmed-article:167189 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:167189 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:167189 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:167189 | pubmed:month | Jun | lld:pubmed |
pubmed-article:167189 | pubmed:issn | 0022-538X | lld:pubmed |
pubmed-article:167189 | pubmed:author | pubmed-author:YoSS | lld:pubmed |
pubmed-article:167189 | pubmed:author | pubmed-author:EmersonS USU | lld:pubmed |
pubmed-article:167189 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:167189 | pubmed:volume | 15 | lld:pubmed |
pubmed-article:167189 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:167189 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:167189 | pubmed:pagination | 1348-56 | lld:pubmed |
pubmed-article:167189 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:167189 | pubmed:year | 1975 | lld:pubmed |
pubmed-article:167189 | pubmed:articleTitle | Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. | lld:pubmed |
pubmed-article:167189 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:167189 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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