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pubmed-article:16716540rdf:typepubmed:Citationlld:pubmed
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pubmed-article:16716540pubmed:dateCreated2006-7-31lld:pubmed
pubmed-article:16716540pubmed:abstractTextFootrot is a mixed bacterial infection of the hooves of sheep. The gram-negative anaerobic bacterium Dichelobacter nodosus is the principal causative agent, with different strains causing diseases of different severity, ranging from benign to virulent. In Australia, in the state of New South Wales (NSW), only virulent footrot is subject to regulatory action, including quarantine. However, it is often difficult to distinguish benign footrot from virulent footrot in the initial stages of infection, or under adverse climatic conditions. The gelatin gel test, which measures the thermostability of secreted bacterial proteases, is the laboratory test most widely used in Australia to aid in the differential diagnosis of footrot. The proteases of virulent strains are, in general, more thermostable than the proteases of benign strains. However, there are some false positives in the gelatin gel test, which may lead to unnecessary quarantine procedures. We used Southern blot analysis on 595 isolates of D. nodosus from 124 farms on which sheep had benign or virulent footrot to test for the presence of the intA gene. We found that for D. nodosus strains which are stable in the gelatin gel test, there is a high correlation between the presence of the intA gene and the ability of the strain to cause virulent footrot. We also developed a PCR-based assay for the rapid detection of intA, which can be used to test DNA extracted from colonies grown on plates, or DNA extracted from cotton swabs of culture plates.lld:pubmed
pubmed-article:16716540pubmed:languageenglld:pubmed
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pubmed-article:16716540pubmed:statusMEDLINElld:pubmed
pubmed-article:16716540pubmed:monthAuglld:pubmed
pubmed-article:16716540pubmed:issn0378-1135lld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:GreenGGlld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:McFarlaneJJlld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:BaileyG DGDlld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:KatzM EMElld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:SutherlandMMlld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:SeamanJ TJTlld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:CheethamB FBFlld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:TanjungL RLRlld:pubmed
pubmed-article:16716540pubmed:authorpubmed-author:DruittJJlld:pubmed
pubmed-article:16716540pubmed:issnTypePrintlld:pubmed
pubmed-article:16716540pubmed:day25lld:pubmed
pubmed-article:16716540pubmed:volume116lld:pubmed
pubmed-article:16716540pubmed:ownerNLMlld:pubmed
pubmed-article:16716540pubmed:authorsCompleteYlld:pubmed
pubmed-article:16716540pubmed:pagination166-74lld:pubmed
pubmed-article:16716540pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:16716540pubmed:meshHeadingpubmed-meshheading:16716540...lld:pubmed
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pubmed-article:16716540pubmed:meshHeadingpubmed-meshheading:16716540...lld:pubmed
pubmed-article:16716540pubmed:year2006lld:pubmed
pubmed-article:16716540pubmed:articleTitleImproved diagnosis of virulent ovine footrot using the intA gene.lld:pubmed
pubmed-article:16716540pubmed:affiliationMolecular and Cellular Biology, University of New England, Armidale, NSW 2351, Australia. bcheetha@une.edu.aulld:pubmed
pubmed-article:16716540pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16716540pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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