| pubmed-article:16698789 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16698789 | lifeskim:mentions | umls-concept:C1704689 | lld:lifeskim |
| pubmed-article:16698789 | lifeskim:mentions | umls-concept:C0001475 | lld:lifeskim |
| pubmed-article:16698789 | lifeskim:mentions | umls-concept:C0376590 | lld:lifeskim |
| pubmed-article:16698789 | lifeskim:mentions | umls-concept:C1710425 | lld:lifeskim |
| pubmed-article:16698789 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:16698789 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:16698789 | pubmed:dateCreated | 2006-5-15 | lld:pubmed |
| pubmed-article:16698789 | pubmed:abstractText | F(1)-ATPase is an ATP-driven rotary molecular motor in which the central gamma-subunit rotates inside a stator cylinder made of alpha(3)beta(3) subunits. To elucidate the role of rotor-stator interactions in torque generation, we truncated the gamma-subunit at its carboxyl terminus, which forms an alpha helix that penetrates deeply into the stator cylinder. We used an alpha(3)beta(3)gamma subcomplex of F(1)-ATPase derived from thermophilic Bacillus PS3 and expressed it in Escherichia coli. We could obtain purified subcomplexes in which 14, 17, or 21 amino-acid residues were deleted. The rotary characteristics of the truncated mutants, monitored by attaching a duplex of 0.49-microm beads to the gamma-subunit, did not differ greatly from those of the wild-type over the ATP concentrations of 20 nM-2 mM, the most conspicuous effect being approximately 50% reduction in torque and approximately 70% reduction in the rate of ATP binding upon deletion of 21 residues. The ATP hydrolysis activity estimated in bulk samples was more seriously affected. The 21-deletion mutant, in particular, was >10-fold less active, but this is likely due to instability of this subcomplex. For torque generation, though not for rapid catalysis, most of the rotor-stator contacts on the deeper half of the penetrating portion of the gamma-subunit are dispensable. | lld:pubmed |
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| pubmed-article:16698789 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:16698789 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16698789 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16698789 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16698789 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:YoshidaMasasu... | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:KinositaKazuh... | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:MakiYasushiY | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:AliM YusufMY | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:AdachiKengoK | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:ItohHiroyasuH | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:FuruikeShouS | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:HossainMohamm... | lld:pubmed |
| pubmed-article:16698789 | pubmed:author | pubmed-author:HuqMominulM | lld:pubmed |
| pubmed-article:16698789 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16698789 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:16698789 | pubmed:volume | 90 | lld:pubmed |
| pubmed-article:16698789 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16698789 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16698789 | pubmed:pagination | 4195-203 | lld:pubmed |
| pubmed-article:16698789 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:16698789 | pubmed:meshHeading | pubmed-meshheading:16698789... | lld:pubmed |
| pubmed-article:16698789 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16698789 | pubmed:articleTitle | The rotor tip inside a bearing of a thermophilic F1-ATPase is dispensable for torque generation. | lld:pubmed |
| pubmed-article:16698789 | pubmed:affiliation | Department of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. | lld:pubmed |
| pubmed-article:16698789 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16698789 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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