| pubmed-article:16682442 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16682442 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:16682442 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:16682442 | lifeskim:mentions | umls-concept:C0035541 | lld:lifeskim |
| pubmed-article:16682442 | lifeskim:mentions | umls-concept:C0449416 | lld:lifeskim |
| pubmed-article:16682442 | lifeskim:mentions | umls-concept:C1622990 | lld:lifeskim |
| pubmed-article:16682442 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:16682442 | lifeskim:mentions | umls-concept:C0073432 | lld:lifeskim |
| pubmed-article:16682442 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:16682442 | pubmed:dateCreated | 2006-5-9 | lld:pubmed |
| pubmed-article:16682442 | pubmed:abstractText | Besides linear RNAs, pre-mRNA splicing generates three forms of RNAs: lariat introns, Y-structure introns from trans-splicing, and circular exons through exon skipping. To study the persistence of excised introns in total cellular RNA, we used three Escherichia coli 3' to 5' exoribonucleases. Ribonuclease R (RNase R) thoroughly degrades the abundant linear RNAs and the Y-structure RNA, while preserving the loop portion of a lariat RNA. Ribonuclease II (RNase II) and polynucleotide phosphorylase (PNPase) also preserve the lariat loop, but are less efficient in degrading linear RNAs. RNase R digestion of the total RNA from human skeletal muscle generates an RNA pool consisting of lariat and circular RNAs. RT-PCR across the branch sites confirmed lariat RNAs and circular RNAs in the pool generated by constitutive and alternative splicing of the dystrophin pre-mRNA. Our results indicate that RNase R treatment can be used to construct an intronic cDNA library, in which majority of the intron lariats are represented. The highly specific activity of RNase R implies its ability to screen for rare intragenic trans-splicing in any target gene with a large background of cis-splicing. Further analysis of the intronic RNA pool from a specific tissue or cell will provide insights into the global profile of alternative splicing. | lld:pubmed |
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| pubmed-article:16682442 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:16682442 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16682442 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16682442 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:16682442 | pubmed:author | pubmed-author:SuzukiHitoshi... | lld:pubmed |
| pubmed-article:16682442 | pubmed:author | pubmed-author:MalhotraArunA | lld:pubmed |
| pubmed-article:16682442 | pubmed:author | pubmed-author:ZuoYuhongY | lld:pubmed |
| pubmed-article:16682442 | pubmed:author | pubmed-author:ZhangMichael... | lld:pubmed |
| pubmed-article:16682442 | pubmed:author | pubmed-author:WangJinhuaJ | lld:pubmed |
| pubmed-article:16682442 | pubmed:author | pubmed-author:MayedaAkilaA | lld:pubmed |
| pubmed-article:16682442 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:16682442 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:16682442 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16682442 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16682442 | pubmed:pagination | e63 | lld:pubmed |
| pubmed-article:16682442 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:meshHeading | pubmed-meshheading:16682442... | lld:pubmed |
| pubmed-article:16682442 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16682442 | pubmed:articleTitle | Characterization of RNase R-digested cellular RNA source that consists of lariat and circular RNAs from pre-mRNA splicing. | lld:pubmed |
