| pubmed-article:16682228 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16682228 | lifeskim:mentions | umls-concept:C0914069 | lld:lifeskim |
| pubmed-article:16682228 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16682228 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:16682228 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:16682228 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16682228 | pubmed:dateCreated | 2006-7-28 | lld:pubmed |
| pubmed-article:16682228 | pubmed:abstractText | Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) is a key enzyme involved in atherosclerosis, and has been considered as a new target for drug discovery. The major difficulty for high-throughput screening of Lp-PLA(2) inhibitors and for functional studies was their fast and efficient production. Purification of native Lp-PLA(2) from human plasma was complicated and produced a very low yield. We herein examined the feasibility of expressing and purifying recombinant Lp-PLA(2) in different heterologous expression systems. The fusion Lp-PLA(2) was expressed at high levels and exhibited strong enzyme activity in insect cell-baculovirus expression system. The functional enzyme could also be produced in Pichia pastoris. The inclusion of a Kozak sequence increased greatly the expression level of recombinant Lp-PLA(2) in insect cells, but had little effect on the expression of recombinant Lp-PLA(2) in P. pastoris and Escherichia coli. P. pastoris-produced Lp-PLA(2) could be purified rapidly and conveniently through a one-step procedure, while baculovirus-produced Lp-PLA(2) could be efficiently purified through a two-step procedure. This ability to readily produce recombinant Lp-PLA(2) could provide a screening model for Lp-PLA(2) inhibitors and will facilitate further studies on this enzyme. | lld:pubmed |
| pubmed-article:16682228 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16682228 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16682228 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16682228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16682228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16682228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16682228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16682228 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16682228 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16682228 | pubmed:issn | 1046-5928 | lld:pubmed |
| pubmed-article:16682228 | pubmed:author | pubmed-author:WangYipingY | lld:pubmed |
| pubmed-article:16682228 | pubmed:author | pubmed-author:DongLiL | lld:pubmed |
| pubmed-article:16682228 | pubmed:author | pubmed-author:ShenJingkangJ | lld:pubmed |
| pubmed-article:16682228 | pubmed:author | pubmed-author:ZhangFujunF | lld:pubmed |
| pubmed-article:16682228 | pubmed:author | pubmed-author:CaiMaojunM | lld:pubmed |
| pubmed-article:16682228 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16682228 | pubmed:volume | 48 | lld:pubmed |
| pubmed-article:16682228 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16682228 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16682228 | pubmed:pagination | 300-6 | lld:pubmed |
| pubmed-article:16682228 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:meshHeading | pubmed-meshheading:16682228... | lld:pubmed |
| pubmed-article:16682228 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16682228 | pubmed:articleTitle | Heterologous expression of lipoprotein-associated phospholipase A2 in different expression systems. | lld:pubmed |
| pubmed-article:16682228 | pubmed:affiliation | State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China. | lld:pubmed |
| pubmed-article:16682228 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16682228 | lld:pubmed |
