| pubmed-article:16679418 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C0242726 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C1095831 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C0004755 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C0018494 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C0009017 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C0445223 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C1552599 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:16679418 | lifeskim:mentions | umls-concept:C1704787 | lld:lifeskim |
| pubmed-article:16679418 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:16679418 | pubmed:dateCreated | 2006-7-7 | lld:pubmed |
| pubmed-article:16679418 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16679418 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16679418 | pubmed:abstractText | Root hairs are specialized epidermal cells that play a role in the uptake of water and nutrients from the rhizosphere and serve as a site of interaction with soil microorganisms. The process of root hair formation is well characterized in Arabidopsis (Arabidopsis thaliana); however, there is a very little information about the genetic and molecular basis of root hair development in monocots. Here, we report on isolation and cloning of the beta-expansin (EXPB) gene HvEXPB1, tightly related to root hair initiation in barley (Hordeum vulgare). Using root transcriptome differentiation in the wild-type/root-hairless mutant system, a cDNA fragment present in roots of wild-type plants only was identified. After cloning of full-length cDNA and genomic sequences flanking the identified fragment, the subsequent bioinformatics analyses revealed homology of the protein coded by the identified gene to the EXPB family. Reverse transcription-PCR showed that expression of HvEXPB1 cosegregated with the root hair phenotype in F2 progeny of the cross between the hairless mutant rhl1.a and the wild-type Karat parent variety. Expression of the HvEXPB1 gene was root specific; it was expressed in roots of wild-type forms, but not in coleoptiles, leaves, tillers, and spikes. The identified gene was active in roots of two other analyzed root hair mutants: rhp1.a developing root hair primordia only and rhs1.a with very short root hairs. Contrary to this, a complete lack of HvEXPB1 expression was observed in roots of the spontaneous root-hairless mutant bald root barley. All these observations suggest a role of the HvEXPB1 gene in the process of root hair formation in barley. | lld:pubmed |
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| pubmed-article:16679418 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16679418 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16679418 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16679418 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16679418 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16679418 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16679418 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:16679418 | pubmed:issn | 0032-0889 | lld:pubmed |
| pubmed-article:16679418 | pubmed:author | pubmed-author:KwasniewskiMi... | lld:pubmed |
| pubmed-article:16679418 | pubmed:author | pubmed-author:SzarejkoIwona... | lld:pubmed |
| pubmed-article:16679418 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16679418 | pubmed:volume | 141 | lld:pubmed |
| pubmed-article:16679418 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16679418 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16679418 | pubmed:pagination | 1149-58 | lld:pubmed |
| pubmed-article:16679418 | pubmed:dateRevised | 2010-9-16 | lld:pubmed |
| pubmed-article:16679418 | pubmed:meshHeading | pubmed-meshheading:16679418... | lld:pubmed |
| pubmed-article:16679418 | pubmed:meshHeading | pubmed-meshheading:16679418... | lld:pubmed |
| pubmed-article:16679418 | pubmed:meshHeading | pubmed-meshheading:16679418... | lld:pubmed |
| pubmed-article:16679418 | pubmed:meshHeading | pubmed-meshheading:16679418... | lld:pubmed |
| pubmed-article:16679418 | pubmed:meshHeading | pubmed-meshheading:16679418... | lld:pubmed |
| pubmed-article:16679418 | pubmed:meshHeading | pubmed-meshheading:16679418... | lld:pubmed |
