| pubmed-article:16666428 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16666428 | lifeskim:mentions | umls-concept:C0016767 | lld:lifeskim |
| pubmed-article:16666428 | lifeskim:mentions | umls-concept:C0242772 | lld:lifeskim |
| pubmed-article:16666428 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:16666428 | lifeskim:mentions | umls-concept:C1167046 | lld:lifeskim |
| pubmed-article:16666428 | lifeskim:mentions | umls-concept:C0039476 | lld:lifeskim |
| pubmed-article:16666428 | lifeskim:mentions | umls-concept:C0439857 | lld:lifeskim |
| pubmed-article:16666428 | lifeskim:mentions | umls-concept:C2263130 | lld:lifeskim |
| pubmed-article:16666428 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:16666428 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:16666428 | pubmed:dateCreated | 2010-6-29 | lld:pubmed |
| pubmed-article:16666428 | pubmed:abstractText | Calcium transport into tomato (Lycopersicon esculentum Mill, cv Castlemart) fruit tonoplast vesicles was studied. Calcium uptake was stimulated approximately 10-fold by MgATP. Two ATP-dependent Ca(2+) transport activities could be resolved on the basis of sensitivity to nitrate and affinity for Ca(2+). A low affinity Ca(2+) uptake system (K(m) > 200 micromolar) was inhibited by nitrate and ionophores and is thought to represent a tonoplast localized H(+)/Ca(2+) antiport. A high affinity Ca(2+) uptake system (K(m) = 6 micromolar) was not inhibited by nitrate, had reduced sensitivity to ionophores, and appeared to be associated with a population of low density endoplasmic reticulum vesicles that contaminated the tonoplast-enriched membrane fraction. Arrhenius plots of the temperature dependence of Ca(2+) transport in tomato membrane vesicles showed a sharp increase in activation energy at temperatures below 10 to 12 degrees C that was not observed in red beet membrane vesicles. This low temperature effect on tonoplast Ca(2+)/H(+) antiport activity could only by partially ascribed to an effect of low temperature on H(+)-ATPase activity, ATP-dependent H(+) transport, passive H(+) fluxes, or passive Ca(2+) fluxes. These results suggest that low temperature directly affects Ca(2+)/H(+) exchange across the tomato fruit tonoplast, resulting in an apparent change in activation energy for the transport reaction. This could result from a direct effect of temperature on the Ca(2+)/H(+) exchange protein or by an indirect effect of temperature on lipid interactions with the Ca(2+)/H(+) exchange protein. | lld:pubmed |
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| pubmed-article:16666428 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16666428 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16666428 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:16666428 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:16666428 | pubmed:issn | 0032-0889 | lld:pubmed |
| pubmed-article:16666428 | pubmed:author | pubmed-author:ReidM SMS | lld:pubmed |
| pubmed-article:16666428 | pubmed:author | pubmed-author:BennettA BAB | lld:pubmed |
| pubmed-article:16666428 | pubmed:author | pubmed-author:JoyceD CDC | lld:pubmed |
| pubmed-article:16666428 | pubmed:author | pubmed-author:CramerG RGR | lld:pubmed |
| pubmed-article:16666428 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16666428 | pubmed:volume | 88 | lld:pubmed |
| pubmed-article:16666428 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16666428 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16666428 | pubmed:pagination | 1097-103 | lld:pubmed |
| pubmed-article:16666428 | pubmed:dateRevised | 2010-9-15 | lld:pubmed |
| pubmed-article:16666428 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:16666428 | pubmed:articleTitle | Transport Properties of the Tomato Fruit Tonoplast : III. Temperature Dependence of Calcium Transport. | lld:pubmed |
| pubmed-article:16666428 | pubmed:affiliation | Department of Environmental Horticulture, University of California, Davis, California 95616. | lld:pubmed |
| pubmed-article:16666428 | pubmed:publicationType | Journal Article | lld:pubmed |
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