| pubmed-article:16661329 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C1138842 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C0242724 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C0033731 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:16661329 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:16661329 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16661329 | pubmed:dateCreated | 2010-6-29 | lld:pubmed |
| pubmed-article:16661329 | pubmed:abstractText | A plasma membrane-enriched fraction has been isolated from corn leaf mesophyll protoplasts and its identity confirmed with the aid of an external label, diazotized [(125)I]iodosulfanilic acid. Gentle cell disruption enabled internal organelles to be maintained intact and thus facilitated separation from the plasma membrane. The plasma membrane-enriched fraction was devoid of chloroplast or mitochondrial markers, whereas markers for the endoplasmic reticulum and golgi indicated minimal contamination. The highly enriched plasma membrane fraction contained a Mg(2+)-dependent, K(+)-stimulated ATPase with a pH optimum near neutrality. The position of the membranes on sucrose density gradients indicates that the plasma membranes have characteristics similar to other plasma membrane fractions. | lld:pubmed |
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| pubmed-article:16661329 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16661329 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16661329 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:16661329 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16661329 | pubmed:issn | 0032-0889 | lld:pubmed |
| pubmed-article:16661329 | pubmed:author | pubmed-author:PerlinD SDS | lld:pubmed |
| pubmed-article:16661329 | pubmed:author | pubmed-author:SpanswickR... | lld:pubmed |
| pubmed-article:16661329 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16661329 | pubmed:volume | 65 | lld:pubmed |
| pubmed-article:16661329 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16661329 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16661329 | pubmed:pagination | 1053-7 | lld:pubmed |
| pubmed-article:16661329 | pubmed:dateRevised | 2010-9-15 | lld:pubmed |
| pubmed-article:16661329 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:16661329 | pubmed:articleTitle | Labeling and isolation of plasma membranes from corn leaf protoplasts. | lld:pubmed |
| pubmed-article:16661329 | pubmed:affiliation | Section of Botany, Genetics and Development, Division of Biological Sciences, Plant Science Building, Cornell University, Ithaca, New York 14853. | lld:pubmed |
| pubmed-article:16661329 | pubmed:publicationType | Journal Article | lld:pubmed |
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