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pubmed-article:16660885pubmed:dateCreated2010-6-29lld:pubmed
pubmed-article:16660885pubmed:abstractTextIntact protoplasts and chloroplasts have been isolated from mature flag leaves of wheat (Triticum aestivum L.). Both showed high rates of photosynthesis, the best of which equaled those observed in the parent tissue (greater than 150 micromoles O(2) per milligram chlorophyll per hour). The presence of ethylenediaminetetraacetate and an alkaline medium (pH 8.4) were required in the isolation and assay for the achievement of maximum rates of photosynthesis by chloroplasts. Photosynthesis by isolated chloroplasts was inhibited at very low concentrations of external orthophosphate.lld:pubmed
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pubmed-article:16660885pubmed:statusPubMed-not-MEDLINElld:pubmed
pubmed-article:16660885pubmed:monthJunlld:pubmed
pubmed-article:16660885pubmed:issn0032-0889lld:pubmed
pubmed-article:16660885pubmed:authorpubmed-author:WalkerD ADAlld:pubmed
pubmed-article:16660885pubmed:authorpubmed-author:LeegoodR CRClld:pubmed
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pubmed-article:16660885pubmed:volume63lld:pubmed
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pubmed-article:16660885pubmed:pagination1212-4lld:pubmed
pubmed-article:16660885pubmed:dateRevised2010-9-15lld:pubmed
pubmed-article:16660885pubmed:year1979lld:pubmed
pubmed-article:16660885pubmed:articleTitleIsolation of Protoplasts and Chloroplasts from Flag Leaves of Triticum aestivum L.lld:pubmed
pubmed-article:16660885pubmed:affiliationDepartment of Botany, The University of Sheffield, Sheffield S10 2TN, England.lld:pubmed
pubmed-article:16660885pubmed:publicationTypeJournal Articlelld:pubmed
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