| pubmed-article:16621790 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16621790 | lifeskim:mentions | umls-concept:C1414536 | lld:lifeskim |
| pubmed-article:16621790 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:16621790 | lifeskim:mentions | umls-concept:C1707310 | lld:lifeskim |
| pubmed-article:16621790 | lifeskim:mentions | umls-concept:C0949455 | lld:lifeskim |
| pubmed-article:16621790 | pubmed:issue | 25 | lld:pubmed |
| pubmed-article:16621790 | pubmed:dateCreated | 2006-6-19 | lld:pubmed |
| pubmed-article:16621790 | pubmed:abstractText | MNSFbeta is a ubiquitously expressed member of the ubiquitin-like family that has been implicated in various biological functions. Previous studies have demonstrated that MNSFbeta covalently binds to intracellular proapoptotic protein Bcl-G in mitogen-activated murine T cells. In this study, we further investigated the intracellular mechanism of action of MNSFbeta in macrophage cell line, Raw 264.7 cells. We present evidence that MNSFbeta.Bcl-G complex associates with ERKs in non-stimulated Raw 264.7. We found that MNSFbeta.Bcl-G directly bound to ERKs and inhibited ERK activation by MEK1. In Raw 264.7 cells treated with MNSFbeta small interfering RNA (siRNA) lipopolysaccharide (LPS)-induced ERK1/2 activation was enhanced and LPS-induced JNK and p38 activation was unaffected. SiRNA-mediated knockdown of MNSFbeta increased tumor necrosis factor alpha (TNFalpha) expression at mRNA and protein levels in LPS-stimulated Raw 264.7 cells. Finally, we found that transfection with MNSFbeta expression construct resulted in a significant inhibition of LPS-induced ERK activation and TNFalpha production. Co-transfection experiments with MNSFbeta and Bcl-G greatly enhanced this inhibition. Collectively, these findings indicate that MNSFbeta might be implicated in the macrophage response to LPS. | lld:pubmed |
| pubmed-article:16621790 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16621790 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16621790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621790 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16621790 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16621790 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:16621790 | pubmed:author | pubmed-author:YamaguchiSeij... | lld:pubmed |
| pubmed-article:16621790 | pubmed:author | pubmed-author:NakamuraMorih... | lld:pubmed |
| pubmed-article:16621790 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16621790 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:16621790 | pubmed:volume | 281 | lld:pubmed |
| pubmed-article:16621790 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16621790 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16621790 | pubmed:pagination | 16861-9 | lld:pubmed |
| pubmed-article:16621790 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:meshHeading | pubmed-meshheading:16621790... | lld:pubmed |
| pubmed-article:16621790 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16621790 | pubmed:articleTitle | The ubiquitin-like protein MNSFbeta regulates ERK-MAPK cascade. | lld:pubmed |
| pubmed-article:16621790 | pubmed:affiliation | Department of Cooperative Medical Research, Collaboration Center, Faculty of Medicine, Shimane University, Izumo, Japan. nkmr0515@med.shimane-u.ac.jp | lld:pubmed |
| pubmed-article:16621790 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:14109 | entrezgene:pubmed | pubmed-article:16621790 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:16621790 | lld:entrezgene |
