| pubmed-article:16621588 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16621588 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:16621588 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:16621588 | lifeskim:mentions | umls-concept:C0011065 | lld:lifeskim |
| pubmed-article:16621588 | lifeskim:mentions | umls-concept:C0029463 | lld:lifeskim |
| pubmed-article:16621588 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:16621588 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:16621588 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:16621588 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16621588 | pubmed:dateCreated | 2006-7-17 | lld:pubmed |
| pubmed-article:16621588 | pubmed:abstractText | The effect of the carcinogen safrole on intracellular Ca2+ movement has not been explored in osteoblast-like cells. This study examined whether safrole could alter Ca2+ handling and viability in MG63 human osteosarcoma cells. Cytosolic free Ca2+ levels ([Ca2+]i) in populations of cells were measured using fura-2 as a fluorescent Ca2+ probe. Safrole at concentrations above 130 microM increased [Ca2+]i in a concentration-dependent manner with an EC50 value of 450 microM. The Ca2+ signal was reduced by 30% by removing extracellular Ca2+. Addition of Ca2+ after safrole had depleted intracellular Ca2+ induced Ca2+ influx, suggesting that safrole caused Ca2+ entry. In Ca2+-free medium, after pretreatment with 650 microM safrole, 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor) failed to release more Ca2+; and pretreatment with thapsigargin inhibited most of the safrole-induced [Ca2+]i increases. Inhibition of phospholipase C with U73122 did not affect safrole-induced Ca2+ release; whereas activation of protein kinase C with phorbol ester enhanced safrole-induced [Ca2+]i increase. Trypan exclusion assays revealed that incubation with 65 microM safrole for 30 min did not kill cells, but incubation with 650 microM safrole for 10-30 min nearly killed all cells. Flow cytometry demonstrated that safrole evoked apoptosis in a concentration-dependent manner. Safrole-induced cytotoxicity was not reversed by chelation of Ca2+ with BAPTA. Collectively, the data suggest that in MG63 cells, safrole induced a [Ca2+]i increase by causing Ca2+ release mainly from the endoplasmic reticulum in a phospholipase C-independent manner. The safrole response involved Ca2+ influx and is modulated by protein kinase C. Furthermore, safrole can cause apoptosis in a Ca2+-independent manner. | lld:pubmed |
| pubmed-article:16621588 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16621588 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16621588 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16621588 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16621588 | pubmed:issn | 1043-6618 | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:ChangHong-Tai... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:JanChung-RenC... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:ChenWei-Chuan... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:HuangJong-Khi... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:HuangChun-Jen... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:LuYih-ChauYC | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:LiuShiuh-InnS... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:ChenI-ShuIS | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:ChenJin-ShyrJ... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:ChengHe-Hsiun... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:HsuShu-ShongS... | lld:pubmed |
| pubmed-article:16621588 | pubmed:author | pubmed-author:LinHsueh-ChiH... | lld:pubmed |
| pubmed-article:16621588 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16621588 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:16621588 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16621588 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16621588 | pubmed:pagination | 103-10 | lld:pubmed |
| pubmed-article:16621588 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:16621588 | pubmed:meshHeading | pubmed-meshheading:16621588... | lld:pubmed |
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| pubmed-article:16621588 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16621588 | pubmed:articleTitle | Safrole-induced cellular Ca2+ increases and death in human osteosarcoma cells. | lld:pubmed |
| pubmed-article:16621588 | pubmed:affiliation | Department of Physical Medicine and Rehabilitation, St. Joseph Hospital, Kaohsiung 802, Taiwan. | lld:pubmed |
| pubmed-article:16621588 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16621588 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
