| pubmed-article:16619302 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16619302 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:16619302 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:16619302 | lifeskim:mentions | umls-concept:C0034809 | lld:lifeskim |
| pubmed-article:16619302 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:16619302 | pubmed:dateCreated | 2006-5-24 | lld:pubmed |
| pubmed-article:16619302 | pubmed:abstractText | The glucocorticoid receptor (GR) acts as a ligand dependent transcription factor but can also cross talk with other signaling pathways via protein-protein interactions. In this paper we describe methods to study novel cytosolic GR interacting proteins, using mAb based immunoaffinity chromatography of GR from rat liver cytosol. Co-purifying proteins were identified by 2-DE in combination with MALDI-TOF-MS. Non-liganded/non-activated and in vitro liganded/activated GR, respectively, co-purifies with specific sets of proteins. Of these 34 were conclusively identified, seven have previously been reported to be part of the GR-complex, revealing 27 new possible interacting candidates for the GR-complex. Of the novel GR interacting proteins the major vault protein, TATA binding interacting protein 49a and glycoprotein PP63 were of special interest. Furthermore, using 2-D DIGE we show that the set of proteins interacting with non-liganded GR is distinctly different in protein amount compared to the proteins found with liganded/activated GR. This suggests the presence of different GR complexes in the cell, which was further substantiated by the finding of several separate GR native protein complexes, "GR-receptosomes", using blue native gel electrophoresis. Our findings suggest the existence of several new mechanisms for GR signaling and regulation. | lld:pubmed |
| pubmed-article:16619302 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16619302 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16619302 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16619302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16619302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16619302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16619302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16619302 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16619302 | pubmed:month | May | lld:pubmed |
| pubmed-article:16619302 | pubmed:issn | 1615-9853 | lld:pubmed |
| pubmed-article:16619302 | pubmed:author | pubmed-author:SchröderWolfg... | lld:pubmed |
| pubmed-article:16619302 | pubmed:author | pubmed-author:GustafssonJan... | lld:pubmed |
| pubmed-article:16619302 | pubmed:author | pubmed-author:WikströmAnn-C... | lld:pubmed |
| pubmed-article:16619302 | pubmed:author | pubmed-author:AsadiAbolfazl... | lld:pubmed |
| pubmed-article:16619302 | pubmed:author | pubmed-author:WidénChristin... | lld:pubmed |
| pubmed-article:16619302 | pubmed:author | pubmed-author:HedmanErikE | lld:pubmed |
| pubmed-article:16619302 | pubmed:author | pubmed-author:DinnetzIngrid... | lld:pubmed |
| pubmed-article:16619302 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16619302 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:16619302 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16619302 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16619302 | pubmed:pagination | 3114-26 | lld:pubmed |
| pubmed-article:16619302 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16619302 | pubmed:meshHeading | pubmed-meshheading:16619302... | lld:pubmed |
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| pubmed-article:16619302 | pubmed:meshHeading | pubmed-meshheading:16619302... | lld:pubmed |
| pubmed-article:16619302 | pubmed:meshHeading | pubmed-meshheading:16619302... | lld:pubmed |
| pubmed-article:16619302 | pubmed:meshHeading | pubmed-meshheading:16619302... | lld:pubmed |
| pubmed-article:16619302 | pubmed:meshHeading | pubmed-meshheading:16619302... | lld:pubmed |
| pubmed-article:16619302 | pubmed:meshHeading | pubmed-meshheading:16619302... | lld:pubmed |
| pubmed-article:16619302 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16619302 | pubmed:articleTitle | Proteomic identification of glucocorticoid receptor interacting proteins. | lld:pubmed |
| pubmed-article:16619302 | pubmed:affiliation | Department of Biosciences and Nutrition, Division of Medical Nutrition, Karolinska Institutet, Novum, S-141 86 Stockholm, Sweden. erik.hedman@mednut.ki.se | lld:pubmed |
| pubmed-article:16619302 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16619302 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:2908 | entrezgene:pubmed | pubmed-article:16619302 | lld:entrezgene |
| entrez-gene:64681 | entrezgene:pubmed | pubmed-article:16619302 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16619302 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16619302 | lld:pubmed |
