| pubmed-article:1660495 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1660495 | lifeskim:mentions | umls-concept:C0022939 | lld:lifeskim |
| pubmed-article:1660495 | lifeskim:mentions | umls-concept:C0317620 | lld:lifeskim |
| pubmed-article:1660495 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:1660495 | lifeskim:mentions | umls-concept:C0009017 | lld:lifeskim |
| pubmed-article:1660495 | lifeskim:mentions | umls-concept:C1882417 | lld:lifeskim |
| pubmed-article:1660495 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:1660495 | pubmed:dateCreated | 1992-1-10 | lld:pubmed |
| pubmed-article:1660495 | pubmed:abstractText | Lactobacillus strain ADH is a bile-resistant, bacteriocin-producing human isolate that was phenotypically classified within the Lactobacillus acidophilus group. Total DNA and phage DNA extracted from strain ADH were separately digested with BclI and ligated with BclI-digested pGK12. Following electroporation of these ligation mixtures directly into strain ADH, electrotransformants were recovered at frequencies of 1.5 x 10(3) and 2.0 x 10(4)/micrograms of pGK12 for preparations of pGK12::phage DNA and pGK12::total DNA, respectively. Among the electrotransformants screened, 6 and 22% contained passenger DNA of either phage DNA or chromosomal origin, respectively, as determined by restriction-enzyme analyses and hybridization assays. Derivatives of pGK12 containing passenger DNA of chromosomal (pTRK120) or phage (pTRK121) origin and pTRK15 (native cryptic plasmid) were evaluated for use as species-specific probes. The strain ADH-derived probes hybridized primarily to members of the B-1 and B-2 lactobacilli homology groups and demonstrated strain-specific polymorphisms within these groups. Identical hybridization patterns were established for strain ADH and Lactobacillus gasseri VPI 6033 (ATCC 19992). Identification of DNA probes and establishment of a host-vector cloning system have facilitated our efforts to characterize the Lactobacillus chromosome and to distinguish between closely related species thought to be important inhabitants of the gastrointestinal tract. | lld:pubmed |
| pubmed-article:1660495 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1660495 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1660495 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1660495 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1660495 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1660495 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1660495 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1660495 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:1660495 | pubmed:issn | 0022-0302 | lld:pubmed |
| pubmed-article:1660495 | pubmed:author | pubmed-author:KlaenhammerT... | lld:pubmed |
| pubmed-article:1660495 | pubmed:author | pubmed-author:TennantM CMC | lld:pubmed |
| pubmed-article:1660495 | pubmed:author | pubmed-author:LuchanskyJ... | lld:pubmed |
| pubmed-article:1660495 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1660495 | pubmed:volume | 74 | lld:pubmed |
| pubmed-article:1660495 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1660495 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1660495 | pubmed:pagination | 3293-302 | lld:pubmed |
| pubmed-article:1660495 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:meshHeading | pubmed-meshheading:1660495-... | lld:pubmed |
| pubmed-article:1660495 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1660495 | pubmed:articleTitle | Molecular cloning and deoxyribonucleic acid polymorphisms in Lactobacillus acidophilus and Lactobacillus gasseri. | lld:pubmed |
| pubmed-article:1660495 | pubmed:affiliation | Southeast Dairy Foods Research Center, North Carolina State University, Raleigh 27695-7624. | lld:pubmed |
| pubmed-article:1660495 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1660495 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1660495 | lld:pubmed |
