| pubmed-article:16584890 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16584890 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:16584890 | lifeskim:mentions | umls-concept:C0079281 | lld:lifeskim |
| pubmed-article:16584890 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16584890 | lifeskim:mentions | umls-concept:C0024485 | lld:lifeskim |
| pubmed-article:16584890 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:16584890 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:16584890 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:16584890 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:16584890 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16584890 | pubmed:dateCreated | 2006-7-28 | lld:pubmed |
| pubmed-article:16584890 | pubmed:abstractText | Human endothelin-1 (ET-1) is a potent vasocontractile 21-residue peptide hormone with significant pharmacological importance. An efficient and straightforward expression strategy that enables cost-effective incorporation of stable isotopes is not available thus far. In this report, we describe a cost-effective expression system in Escherichia coli for the production of ET-1 enriched with (15)N and (13)C isotopes. Employing thioredoxin as carrier protein, specific and nearly quantitative cleavage of ET-1 from the fusion was mediated by Factor Xa, and purification to homogeneity (final purity of >95%) was achieved by RP-HPLC. Purified recombinant ET-1 was found to be indistinguishable from the synthetic counterpart as determined by mass spectrometry and NMR spectroscopy. Our expression strategy offers the potential for production of isotopically labeled ET-1 in large (mg) quantities for the purpose of heteronuclear NMR experiments. Moreover, the method devised should be applicable for recombinant expression of small peptides in general. | lld:pubmed |
| pubmed-article:16584890 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16584890 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16584890 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16584890 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16584890 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16584890 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16584890 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16584890 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16584890 | pubmed:issn | 1046-5928 | lld:pubmed |
| pubmed-article:16584890 | pubmed:author | pubmed-author:BeyermannMich... | lld:pubmed |
| pubmed-article:16584890 | pubmed:author | pubmed-author:OschkinatHart... | lld:pubmed |
| pubmed-article:16584890 | pubmed:author | pubmed-author:SchmiederPete... | lld:pubmed |
| pubmed-article:16584890 | pubmed:author | pubmed-author:PiresJosé... | lld:pubmed |
| pubmed-article:16584890 | pubmed:author | pubmed-author:MacThien-ThiT... | lld:pubmed |
| pubmed-article:16584890 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16584890 | pubmed:volume | 48 | lld:pubmed |
| pubmed-article:16584890 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16584890 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16584890 | pubmed:pagination | 253-60 | lld:pubmed |
| pubmed-article:16584890 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:meshHeading | pubmed-meshheading:16584890... | lld:pubmed |
| pubmed-article:16584890 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16584890 | pubmed:articleTitle | High yield expression and purification of isotopically labelled human endothelin-1 for use in NMR studies. | lld:pubmed |
| pubmed-article:16584890 | pubmed:affiliation | Abt. NMR-unterstützte Strukturbiologie, Forschungsinstitut für molekulare Pharmakologie, Berlin, Germany. | lld:pubmed |
| pubmed-article:16584890 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16584890 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
