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pubmed-article:1658221pubmed:abstractTextThe pathology of Coxsackie virus B4 (CVB4) infection in a murine model was investigated by in situ hybridisation using a biotinylated cDNA probe derived from CVB4. During the acute phase of infection virus RNA sequences were detected in the exocrine pancreas of 60% of mice infected with a pancreotropic variant of CVB4. A positive hybridisation signal was observed in other organs in some animals including the heart and liver of 1 mouse 28 days after infection. The cellular distribution of virus RNA sequences corresponded well with the histological findings in most tissues. Possible causes for failure of hybridisation in some infected pancreases are discussed in conjunction with potential application of the technique in human pancreas biopsy samples.lld:pubmed
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pubmed-article:1658221pubmed:dateRevised2003-11-14lld:pubmed
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pubmed-article:1658221pubmed:year1991lld:pubmed
pubmed-article:1658221pubmed:articleTitleCoxsackie virus B4 infection of the mouse pancreas: I. Detection of virus-specific RNA in the pancreas by in situ hybridisation.lld:pubmed
pubmed-article:1658221pubmed:affiliationDepartment of Immunology, Royal London Hospital Medical College, England.lld:pubmed
pubmed-article:1658221pubmed:publicationTypeJournal Articlelld:pubmed