| pubmed-article:16571826 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C0702091 | lld:lifeskim |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C1007998 | lld:lifeskim |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C0010546 | lld:lifeskim |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C0205164 | lld:lifeskim |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C1705241 | lld:lifeskim |
| pubmed-article:16571826 | lifeskim:mentions | umls-concept:C1705242 | lld:lifeskim |
| pubmed-article:16571826 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:16571826 | pubmed:dateCreated | 2006-3-30 | lld:pubmed |
| pubmed-article:16571826 | pubmed:abstractText | The linear, positive-stranded RNA genome of flaviviruses is thought to adopt a circularized conformation via interactions of short complementary sequence elements located within its terminal regions. This process of RNA cyclization is a crucial precondition for RNA replication. In the case of mosquito-borne flaviviruses, highly conserved cyclization sequences (CS) have been identified, and their functionality has been experimentally confirmed. Here, we provide an experimental identification of CS elements of tick-borne encephalitis virus (TBEV). These elements, termed 5'-CS-A and 3'-CS-A, are conserved among various tick-borne flaviviruses, but they are unrelated to the mosquito-borne CS elements and are located at different genomic positions. The 5'-CS-A element is situated upstream rather than downstream of the AUG start codon and, in contrast to mosquito-borne flaviviruses, it was found that the entire protein C coding region is not essential for TBEV replication. The complementary 3'-CS-A element is located within the bottom stem rather than upstream of the characteristic 3'-terminal stem-loop structure, implying that this part of the proposed structure cannot be formed when the genome is in its circularized conformation. Finally, we demonstrate that the CS-A elements can also mediate their function when the 5'-CS-A element is moved from its natural position to one corresponding to the mosquito-borne CS. The recognition of essential RNA elements and their differences between mosquito-borne and tick-borne flaviviruses has practical implications for the design of replicons in vaccine and vector development. | lld:pubmed |
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| pubmed-article:16571826 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16571826 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16571826 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16571826 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16571826 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16571826 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:16571826 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:16571826 | pubmed:author | pubmed-author:KoflerRegina... | lld:pubmed |
| pubmed-article:16571826 | pubmed:author | pubmed-author:MandlChristia... | lld:pubmed |
| pubmed-article:16571826 | pubmed:author | pubmed-author:ThurnerCaroli... | lld:pubmed |
| pubmed-article:16571826 | pubmed:author | pubmed-author:HoenningerVer... | lld:pubmed |
| pubmed-article:16571826 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16571826 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:16571826 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16571826 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16571826 | pubmed:pagination | 4099-113 | lld:pubmed |
| pubmed-article:16571826 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:16571826 | pubmed:meshHeading | pubmed-meshheading:16571826... | lld:pubmed |
| pubmed-article:16571826 | pubmed:meshHeading | pubmed-meshheading:16571826... | lld:pubmed |
| pubmed-article:16571826 | pubmed:meshHeading | pubmed-meshheading:16571826... | lld:pubmed |
| pubmed-article:16571826 | pubmed:meshHeading | pubmed-meshheading:16571826... | lld:pubmed |
| pubmed-article:16571826 | pubmed:meshHeading | pubmed-meshheading:16571826... | lld:pubmed |
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| pubmed-article:16571826 | pubmed:meshHeading | pubmed-meshheading:16571826... | lld:pubmed |
