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pubmed-article:1656012pubmed:abstractTextMelatonin binding was localized to the inner plexiform layer (IPL) of the frog retina by in vitro autoradiography, using 2-125I-melatonin as the radioligand. Radioreceptor binding assays of frog retinal homogenate demonstrated saturable melatonin binding. Scatchard analysis revealed a single population of binding sites with an apparent dissociation constant (Kd) of 125 pM, with a Bmax of 0.138 fmoles/mg of protein. These results suggest that high-affinity melatonin binding sites are present in the IPL of the frog retina, which may reflect the presence of melatonin receptors in this synaptic layer.lld:pubmed
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pubmed-article:1656012pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:1656012pubmed:articleTitleLocalization and quantification of high-affinity melatonin binding sites in Rana pipiens retina.lld:pubmed
pubmed-article:1656012pubmed:affiliationDepartment of Neurobiology and Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1010.lld:pubmed
pubmed-article:1656012pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1656012pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed