| pubmed-article:1654478 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1654478 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:1654478 | lifeskim:mentions | umls-concept:C1522424 | lld:lifeskim |
| pubmed-article:1654478 | lifeskim:mentions | umls-concept:C0687028 | lld:lifeskim |
| pubmed-article:1654478 | lifeskim:mentions | umls-concept:C0007776 | lld:lifeskim |
| pubmed-article:1654478 | lifeskim:mentions | umls-concept:C0022655 | lld:lifeskim |
| pubmed-article:1654478 | lifeskim:mentions | umls-concept:C0001613 | lld:lifeskim |
| pubmed-article:1654478 | lifeskim:mentions | umls-concept:C1516698 | lld:lifeskim |
| pubmed-article:1654478 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:1654478 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:1654478 | pubmed:dateCreated | 1991-10-24 | lld:pubmed |
| pubmed-article:1654478 | pubmed:abstractText | A cortical collecting duct (CCD) cell line has been developed from a mouse transgenic for the early region of simian virus 40, Tg(SV40E)Bri/7. CCDs were microdissected and placed on collagen gels. Monolayers were subsequently subcultured onto permeable collagen membranes and maintained in serum-supplemented medium. One line, designated M-1, retained many characteristics of the CCD, including a typical epithelial appearance and CCD-specific antigens. M-1 cells, when grown in monolayers on permeable supports, exhibited a high transepithelial resistance (885.7 +/- 109.6 ohms/cm2) and developed a lumen negative transepithelial potential difference (PD) of -45.7 +/- 3.5 mV. The associated short-circuit current (SCC) averaged 71.8 +/- 10.3 microA/cm2, and was reduced by 95% by luminal application of amiloride. The cultured cells responded to arginine vasopressin (AVP) with a significant increase in SCC. M-1 cells generated significant transepithelial solute gradients. After 24 hours incubation, the composition of the luminal (L) and basolateral (B) media (in mM) was: [Na+], L = 106.7 +/- 0.9 and B = 127.4 +/- 0.4; [K+], L = 8.6 +/- 0.6 and B = 2.1 +/- 0.3; [Cl], L = 68.6 +/- 5.8 and B = 101.8 +/- 6.6; [HCO3], L = 15.5 +/- 1.5 and B = 8.6 +/- 1.2; while pH was 7.16 +/- 0.03 at the luminal and 6.94 +/- 0.03 at the basolateral side. The formation of these concentration gradients indicates that the CCD cultures absorb Na+ and Cl- and secrete K+.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
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| pubmed-article:1654478 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1654478 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1654478 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1654478 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1654478 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:1654478 | pubmed:issn | 0085-2538 | lld:pubmed |
| pubmed-article:1654478 | pubmed:author | pubmed-author:ItoSS | lld:pubmed |
| pubmed-article:1654478 | pubmed:author | pubmed-author:CarreteroO... | lld:pubmed |
| pubmed-article:1654478 | pubmed:author | pubmed-author:Fejes-TóthGG | lld:pubmed |
| pubmed-article:1654478 | pubmed:author | pubmed-author:Náray-Fejes-T... | lld:pubmed |
| pubmed-article:1654478 | pubmed:author | pubmed-author:StoosB ABA | lld:pubmed |
| pubmed-article:1654478 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1654478 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:1654478 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1654478 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1654478 | pubmed:pagination | 1168-75 | lld:pubmed |
| pubmed-article:1654478 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:1654478 | pubmed:meshHeading | pubmed-meshheading:1654478-... | lld:pubmed |
| pubmed-article:1654478 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1654478 | pubmed:articleTitle | Characterization of a mouse cortical collecting duct cell line. | lld:pubmed |
| pubmed-article:1654478 | pubmed:affiliation | Henry Ford Hospital, Hypertension Research Division, Detroit, Michigan. | lld:pubmed |
| pubmed-article:1654478 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1654478 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1654478 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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