| pubmed-article:1653704 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1653704 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1653704 | lifeskim:mentions | umls-concept:C0079323 | lld:lifeskim |
| pubmed-article:1653704 | lifeskim:mentions | umls-concept:C0029446 | lld:lifeskim |
| pubmed-article:1653704 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1653704 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1653704 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:1653704 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1653704 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1653704 | pubmed:dateCreated | 1991-10-15 | lld:pubmed |
| pubmed-article:1653704 | pubmed:abstractText | A cDNA construct (approximately 1 kb) of human BM-40 in a plasmid with the cytomegalovirus promoter and enhancer was used to produce several stable clones by transfecting two human cell lines (293, HT 1080). These clones showed a high expression of exogenous 1-kb BM-40 mRNA and no or only little endogenous 2.2-kb mRNA. These clones also secreted BM-40 at high rates (5-50 micrograms ml-1 day-1) into serum-free culture medium as shown by electrophoresis, radioimmunoassay and metabolic labelling. Transfection with the plasmid and overexpression of BM-40 had no effect on cell spreading, proliferation rate and adhesion patterns to extracellular matrix substrates. Recombinant human BM-40 was purified by anion-exchange chromatography and showed the expected N-terminal sequence and amino acid composition. The protein was also identical or similar to authentic BM-40 purified from the mouse Engelbreth-Holm-Swarm tumor in hexosamine content, electrophoretic mobility, circular dichroism and binding activity for calcium and collagen IV. Reduction of both authentic and recombinant BM-40 decreased binding activity which indicates correct formation of disulfide bonds in the recombinant protein. A specific and sensitive radioimmunoassay for human BM-40 was shown to be useful for detecting small quantities of the protein in human cell culture medium and blood. No significant cross-reaction was, however, detected between human and mouse BM-40. | lld:pubmed |
| pubmed-article:1653704 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1653704 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1653704 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1653704 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1653704 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1653704 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:1653704 | pubmed:author | pubmed-author:TimplRR | lld:pubmed |
| pubmed-article:1653704 | pubmed:author | pubmed-author:MayerUU | lld:pubmed |
| pubmed-article:1653704 | pubmed:author | pubmed-author:AumailleyMM | lld:pubmed |
| pubmed-article:1653704 | pubmed:author | pubmed-author:KriegTT | lld:pubmed |
| pubmed-article:1653704 | pubmed:author | pubmed-author:NischtRR | lld:pubmed |
| pubmed-article:1653704 | pubmed:author | pubmed-author:PottgiesserJJ | lld:pubmed |
| pubmed-article:1653704 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1653704 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:1653704 | pubmed:volume | 200 | lld:pubmed |
| pubmed-article:1653704 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1653704 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1653704 | pubmed:pagination | 529-36 | lld:pubmed |
| pubmed-article:1653704 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:1653704 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1653704 | pubmed:articleTitle | Recombinant expression and properties of the human calcium-binding extracellular matrix protein BM-40. | lld:pubmed |
| pubmed-article:1653704 | pubmed:affiliation | Department of Dermatology, University of Munich, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:1653704 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1653704 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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