Linked Life Data

16535458

Source:http://linkedlifedata.com/resource/pubmed/id/16535458

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SubjectPredicateObjectContext
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pubmed-article:16535458pubmed:issue12lld:pubmed
pubmed-article:16535458pubmed:dateCreated2010-6-25lld:pubmed
pubmed-article:16535458pubmed:abstractTextMineralization of pentachlorophenol (PCP) was studied in nonsterile soil from a PCP-contaminated site upon inoculation with two PCP-degrading bacterial strains. At spiked [(sup14)C]PCP concentrations of 30 and 100 mg/kg, the effects of organism type, different inoculation techniques, including structural amendment with sawdust and cell attachment to polyurethane (PU), as well as the effect of different inoculum sizes of 10(sup4) to 10(sup8) cells per g (dry weight) of soil were compared with PCP mineralization by indigenous bacteria. Gas chromatographic analysis was used to monitor PCP disappearance and to check mass balances. The survival and activity of the released bacteria were examined by immunofluorescence microscopy and respiking experiments. Noninoculated soil completely mineralized 30 mg of PCP per kg within 7 months but showed no or only low degradation activity at 100 mg/kg in the same period. Structural amendment with PU or sawdust initiated slow mineralization after half a year. Soil inoculation with Sphingomonas chlorophenolica RA2 shortened the mineralization time drastically to 1 month at 30 mg of PCP per kg using 10(sup8) cells per g, with approximately 80% of the added radioactivity being converted to CO(inf2). The inoculated cells disappeared rapidly, with a count of 2 x 10(sup6) cells per g after 2.3 months and nondetectability after 7 months. At 100 mg/kg, mineralization was slower because of PCP toxicity but approached completion within 7.5 months. The inhibition could be overcome by addition of sawdust (1 g/kg of soil), resulting in a mineralization rate of 3 to 4 mg/kg(middot)d. PU had the opposite effect. Lower inoculum densities resulted in prolonged lag phases and lower rates, although mineralization was still enhanced over the background level. At 30 mg of PCP per kg, inoculation with Mycobacterium chlorophenolicum PCP1 increased mineralization slightly over the indigenous bacterial activity, regardless of inoculum size, but only when the organisms were attached to PU. At 100 mg of PCP per kg, only 27% were mineralized within 7.5 months. After 7 months, the original strain PCP1 inoculum of 10(sup8) cells per g was recovered at 5 x 10(sup6) to 3 x 10(sup7) cells per g, depending on the PCP concentration, but independent of PU amendment. Amendment with sawdust had no effect on the performance of this organism. Possible reasons for the poor performance of this strain include its sensitivity to PCP and its preference for slightly acidic soil conditions.lld:pubmed
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pubmed-article:16535458pubmed:statusPubMed-not-MEDLINElld:pubmed
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pubmed-article:16535458pubmed:issn0099-2240lld:pubmed
pubmed-article:16535458pubmed:authorpubmed-author:KarlsonUUlld:pubmed
pubmed-article:16535458pubmed:authorpubmed-author:MiethlingRRlld:pubmed
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pubmed-article:16535458pubmed:volume62lld:pubmed
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pubmed-article:16535458pubmed:pagination4361-6lld:pubmed
pubmed-article:16535458pubmed:dateRevised2010-9-15lld:pubmed
pubmed-article:16535458pubmed:year1996lld:pubmed
pubmed-article:16535458pubmed:articleTitleAccelerated Mineralization of Pentachlorophenol in Soil upon Inoculation with Mycobacterium chlorophenolicum PCP1 and Sphingomonas chlorophenolica RA2.lld:pubmed
pubmed-article:16535458pubmed:publicationTypeJournal Articlelld:pubmed
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