| pubmed-article:1653025 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C0227525 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C0022173 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C0547047 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C1705241 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C1705242 | lld:lifeskim |
| pubmed-article:1653025 | lifeskim:mentions | umls-concept:C0070750 | lld:lifeskim |
| pubmed-article:1653025 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1653025 | pubmed:dateCreated | 1991-10-9 | lld:pubmed |
| pubmed-article:1653025 | pubmed:abstractText | Two main forms of protein kinase C (PKC) activity were found in rat hepatocytes using DEAE-cellulose chromatography: PKC 1 and PKC 2. Treatment of cells with 1 microM 12-O-tetradecanoylphorbol 13-acetate (TPA) for 15 min caused a marked loss of PKC 1 activity and only a small loss of PKC 2 activity. Hydroxyapatite column chromatography resolved PKC 1 into three distinct peaks 1a, 1b and 1c, and PKC 2 into four peaks 2a, 2b, 2c and 2d. Immunoblot analysis with isozyme-specific monoclonal antibodies identified peak 1a as PKC-beta and peak 1b as PKC-alpha; the other peaks of activity were not identified. Treatment with TPA provoked a loss of activity of peaks 1b (PKC-alpha) and 1c, whereas peak 1a (PKC-beta) activity was not affected. The peaks of activity corresponding to PCK 2 did not show any major change due to TPA treatment except peak 2d that decreased. The apparent disappearance of PKC histone-kinase activity induced by TPA was also observed using other substrates (protamine or vinculin). The TPA-induced decrease in activity occurs in a time-dependent and dose-dependent fashion. However, the time-courses, the extent of depletion and the potency order of phorbol esters in induction of an activity decrease in the two groups of isoforms exhibited substantial differences. | lld:pubmed |
| pubmed-article:1653025 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1653025 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1653025 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1653025 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1653025 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1653025 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1653025 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1653025 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1653025 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1653025 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:1653025 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:1653025 | pubmed:author | pubmed-author:García-SáinzJ... | lld:pubmed |
| pubmed-article:1653025 | pubmed:author | pubmed-author:Robles-Flores... | lld:pubmed |
| pubmed-article:1653025 | pubmed:author | pubmed-author:Alcántara-Her... | lld:pubmed |
| pubmed-article:1653025 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1653025 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:1653025 | pubmed:volume | 1094 | lld:pubmed |
| pubmed-article:1653025 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1653025 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1653025 | pubmed:pagination | 77-84 | lld:pubmed |
| pubmed-article:1653025 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:meshHeading | pubmed-meshheading:1653025-... | lld:pubmed |
| pubmed-article:1653025 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1653025 | pubmed:articleTitle | Differences in phorbol ester-induced decrease of the activity of protein kinase C isozymes in rat hepatocytes. | lld:pubmed |
| pubmed-article:1653025 | pubmed:affiliation | Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, D.F. | lld:pubmed |
| pubmed-article:1653025 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1653025 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1653025 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1653025 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1653025 | lld:pubmed |
