| pubmed-article:16521164 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C1708335 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0006992 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0039789 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0599748 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0524527 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:16521164 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:16521164 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:16521164 | pubmed:dateCreated | 2006-4-17 | lld:pubmed |
| pubmed-article:16521164 | pubmed:abstractText | A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method to determine carbocysteine in human plasma was developed and fully validated. After methanol-induced protein precipitation of the plasma samples, carbocysteine was subjected to LC/MS/MS analysis using electrospray ionization (ESI). The MS system was operated in the selected ion monitoring (SRM) mode. Chromatographic separation was performed on a Hypurity C18 column (i.d. 2.1 mm x 50 mm, particle size 5 microm). The method had a chromatographic running time of 2.0 min and linear calibration curves over the concentration ranges of 0.1-20 microg/mL for carbocysteine. The lower limit of quantification (LLOQ) of the method was 0.1 microg/mL for carbocysteine. The intra- and inter-day precision was less than 7% for all quality control samples at concentrations of 0.5, 2.0, and 10.0 microg/mL. These results indicate that the method was efficient with a simple preparation procedure and a very short running time (2.0 min) for carbocysteine compared with methods reported in the literature and had high selectivity, acceptable accuracy, precision and sensitivity. The validated LC/MS/MS method has been successfully used to a bioequivalence study of two tablet formulations of carbocysteine in healthy volunteers. | lld:pubmed |
| pubmed-article:16521164 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16521164 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16521164 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16521164 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16521164 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16521164 | pubmed:issn | 0951-4198 | lld:pubmed |
| pubmed-article:16521164 | pubmed:author | pubmed-author:HuangMinM | lld:pubmed |
| pubmed-article:16521164 | pubmed:author | pubmed-author:ZhouShufengS | lld:pubmed |
| pubmed-article:16521164 | pubmed:author | pubmed-author:HanJ-HJH | lld:pubmed |
| pubmed-article:16521164 | pubmed:author | pubmed-author:ChenXiaoX | lld:pubmed |
| pubmed-article:16521164 | pubmed:author | pubmed-author:DengYingY | lld:pubmed |
| pubmed-article:16521164 | pubmed:author | pubmed-author:ZhongGuo-ping... | lld:pubmed |
| pubmed-article:16521164 | pubmed:author | pubmed-author:BiHui-ChangHC | lld:pubmed |
| pubmed-article:16521164 | pubmed:author | pubmed-author:ZhaoLi-ziLZ | lld:pubmed |
| pubmed-article:16521164 | pubmed:copyrightInfo | Copyright 2006 John Wiley & Sons, Ltd. | lld:pubmed |
| pubmed-article:16521164 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16521164 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:16521164 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16521164 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16521164 | pubmed:pagination | 1153-60 | lld:pubmed |
| pubmed-article:16521164 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16521164 | pubmed:meshHeading | pubmed-meshheading:16521164... | lld:pubmed |
| pubmed-article:16521164 | pubmed:meshHeading | pubmed-meshheading:16521164... | lld:pubmed |
| pubmed-article:16521164 | pubmed:meshHeading | pubmed-meshheading:16521164... | lld:pubmed |
| pubmed-article:16521164 | pubmed:meshHeading | pubmed-meshheading:16521164... | lld:pubmed |
| pubmed-article:16521164 | pubmed:meshHeading | pubmed-meshheading:16521164... | lld:pubmed |
| pubmed-article:16521164 | pubmed:meshHeading | pubmed-meshheading:16521164... | lld:pubmed |
| pubmed-article:16521164 | pubmed:meshHeading | pubmed-meshheading:16521164... | lld:pubmed |
| pubmed-article:16521164 | pubmed:meshHeading | pubmed-meshheading:16521164... | lld:pubmed |
| pubmed-article:16521164 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16521164 | pubmed:articleTitle | High-throughput determination of carbocysteine in human plasma by liquid chromatography/tandem mass spectrometry: application to a bioequivalence study of two formulations in healthy volunteers. | lld:pubmed |
| pubmed-article:16521164 | pubmed:affiliation | Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, China. | lld:pubmed |
| pubmed-article:16521164 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16521164 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16521164 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| pubmed-article:16521164 | pubmed:publicationType | Validation Studies | lld:pubmed |
