| pubmed-article:1651770 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1651770 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1651770 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:1651770 | lifeskim:mentions | umls-concept:C0021756 | lld:lifeskim |
| pubmed-article:1651770 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:1651770 | lifeskim:mentions | umls-concept:C0031437 | lld:lifeskim |
| pubmed-article:1651770 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:1651770 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:1651770 | lifeskim:mentions | umls-concept:C0443252 | lld:lifeskim |
| pubmed-article:1651770 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:1651770 | pubmed:dateCreated | 1991-9-23 | lld:pubmed |
| pubmed-article:1651770 | pubmed:abstractText | We have studied growth, function, and phenotype of purified NK and T cells in long-term cultures and compared these parameters to those of conventionally prepared interleukin-2-activated lymphocytes with killer cell activity (LAK). Enrichment of NK and T cells was achieved by Percoll density gradient. Growth was analyzed by cell counts and [3H]TdR uptake. Cytotoxicity and tumor-binding efficacy were assessed in a 3-h 51Cr and single cell agarose assay, respectively, against K-562, Daudi, human ovarian cell line Ovcar-3, and fresh leukemic blasts. We found that long-term proliferation and lytic activity were highest in NK-enriched and lowest in T-enriched cultures. Conventional LAK cultures generated medium cytotoxicity levels. Lytic activity declined within 3 weeks in cultures not enriched for NK cells, while NK-enriched cultures showed high levels of cytotoxicity up to 6 weeks. No change was found in binding activity within 3 weeks with the exception of T cell-enriched fraction. A number of changes in the phenotypic patterns was observed in IL-2 cultures; the CD56+/CD3-/+ and CD56+/CD8+ subset increased in most cultures, whereas the CD56-/CD3+ subset decreased over time. The highly enriched NK cell culture maintained its NK cell phenotype over 5-6 weeks. We also delineated the most cytotoxic lymphocyte subset in long-term IL-2 cultures by complement dependent cytotoxic assays and fluorescence-activated cell sorting (FACS). Lytic activity in conventional LAK as well as in T and NK cell-enriched IL-2 cultures was mediated primarily by CD56+, CD16+, CD3- NK cells. The clinical implication of these studies is discussed. | lld:pubmed |
| pubmed-article:1651770 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1651770 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1651770 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1651770 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1651770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1651770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1651770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1651770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1651770 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1651770 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1651770 | pubmed:issn | 1056-5477 | lld:pubmed |
| pubmed-article:1651770 | pubmed:author | pubmed-author:LotzováEE | lld:pubmed |
| pubmed-article:1651770 | pubmed:author | pubmed-author:FuchshuberP... | lld:pubmed |
| pubmed-article:1651770 | pubmed:author | pubmed-author:PollockR ERE | lld:pubmed |
| pubmed-article:1651770 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1651770 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:1651770 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1651770 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1651770 | pubmed:pagination | 51-9 | lld:pubmed |
| pubmed-article:1651770 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:meshHeading | pubmed-meshheading:1651770-... | lld:pubmed |
| pubmed-article:1651770 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1651770 | pubmed:articleTitle | Antitumor activity, growth, and phenotype of long-term IL-2 cultures of human NK and T lymphocytes. | lld:pubmed |
| pubmed-article:1651770 | pubmed:affiliation | Department of General Surgery, University of Texas, M.D. Anderson Cancer Center, Houston 77030. | lld:pubmed |
| pubmed-article:1651770 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1651770 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1651770 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1651770 | lld:pubmed |
